Natural variation in wild tomato trichomes; selecting metabolites that contribute to insect resistance using a random forest approach
| Autor: | Robert C. Schuurink, Bart André, Frans M. van der Kloet, Sacha A. F. T. van Hijum, Aleksandra Muras, Petra M. Bleeker, Michel A. Haring, Maurice Heilijgers, Marc Galland, Ruy Kortbeek |
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| Přispěvatelé: | Plant Physiology (SILS, FNWI), Biosystems Data Analysis (SILS, FNWI) |
| Rok vydání: | 2021 |
| Předmět: |
Volatiles
0106 biological sciences 0301 basic medicine Genotype media_common.quotation_subject Thrips Plant Science Whitefly Insect Biology Solanum 01 natural sciences Tomato Hemiptera 03 medical and health sciences Acylsugar Botany Animals Wild tomato Cultivar Acylsugars Insect resistance Disease Resistance media_common Ecotype Volatile Organic Compounds Research Thysanoptera fungi Genetic Variation food and beverages Trichomes biology.organism_classification Trichome Western flower thrips Specialised metabolites Phenotype 030104 developmental biology QK1-989 Metabolome Algorithms Random forest 010606 plant biology & botany |
| Zdroj: | BMC Plant Biology, Vol 21, Iss 1, Pp 1-19 (2021) BMC Plant Biology BMC plant biology, 21:315. BioMed Central |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/s12870-021-03070-x |
| Popis: | BackgroundPlant-produced specialised metabolites are a powerful part of a plant’s first line of defence against herbivorous insects, bacteria and fungi. Wild ancestors of present-day cultivated tomato produce a plethora of acylsugars in their type-I/IV trichomes and volatiles in their type-VI trichomes that have a potential role in plant resistance against insects. However, metabolic profiles are often complex mixtures making identification of the functionally interesting metabolites challenging. Here, we aimed to identify specialised metabolites from a wide range of wild tomato genotypes that could explain resistance to vector insects whitefly (Bemisia tabaci) and Western flower thrips (Frankliniella occidentalis). We evaluated plant resistance,determined trichome density and obtained metabolite profiles of the glandular trichomes by LC-MS (acylsugars) and GC-MS (volatiles). Using a customised Random Forest learning algorithm, we determined the contribution of specific specialised metabolites to the resistance phenotypes observed.ResultsThe selected wild tomato accessions showed different levels of resistance to both whiteflies and thrips. Accessions resistant to one insect can be susceptible to another. Glandular trichome density is not necessarily a good predictor for plant resistance although the density of type-I/IV trichomes, related to the production of acylsugars, appears to correlate with whitefly resistance. For type VI-trichomes, however, it seems resistance is determined by the specific content of the glands. There is a strong qualitative and quantitative variation in the metabolite profiles between different accessions, even when they are from the same species. Out of 76 acylsugars found, the random forest algorithm linked two acylsugars (S3:15 and S3:21) to whitefly resistance, but none to thrips resistance. Out of 86 volatiles detected, the sesquiterpene α-humulene was linked to whitefly susceptible accessions instead. The algorithm did not link any specific metabolite to resistance against thrips, but monoterpenes α-phellandrene, α-terpinene and β-phellandrene/D-limonene were significantly associated with susceptible tomato accessions.ConclusionsWhiteflies and thrips are distinctly targeted by certain specialised metabolites found in wild tomatoes. The machine learning approach presented helped to identify features with efficacy toward the insect species studied. These acylsugar metabolites can be targets for breeding efforts towards the selection of insect-resistant cultivars. |
| Databáze: | OpenAIRE |
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