Evaluation of the stability and efficacy of a propofol-rocuronium mixture

Autor: Sevim Rollas, Ziya Salihoglu, Arzu Duran, Saffet Karaca
Rok vydání: 2003
Předmět:
Zdroj: Canadian journal of anaesthesia = Journal canadien d'anesthesie. 50(3)
ISSN: 0832-610X
Popis: To the Editor: Alternative strategies to reduce the onset time of nondepolarizing muscle relaxants, such as priming,1 administering large doses2 and using the timing principle3,4 have not been completely succesful. We have used a propofolrocuronium mixture for the rapid induction of anesthesia. First however, we tested whether a propofol-rocuronium mixture is stable. UV spectra were obtained on a Shimadzu UV 2100 sec spectrophotometer (10 mg·100 mL–1 in acetonitrile). A model 600 Waters pump was connected to a 200 μL loop injector and a μBondapak CN column (150 mm × 3.9 mm internal diameter; Waters assoc. Milford, MA, USA). Model 481 Waters UV detector and Unicam ProGC Data Station were used. Compounds were detected at 220 nm. The peak height was used for quantitation. The mobile phase was acetonitrile-water (60:40, v/v). The flow rate, injection volume and detector response were 0.7 mL·mL–1, 5 μL and 15 μL and 100 mV, respectively. High pressure liquid chromatography (HPLC) method: standard stock solutions were freshly prepared with acetonitrile including 60 μg·mL–1 rocuronium bromide and 100 μg·mL–1 propofol. The external standard solutions were prepared with acetonitrile at a concentration of 60 μg·mL–1 for rocuronium bromide and at a concentration of 100 μg·mL–1 for propofol. Standard propofol peak height with a standard deviation of 0.38 and standard rocuronium bromide peak height with a standard deviation of 0.25 were established. Peak heights of propofol and rocuronium bromide in propofol-rocuronium bromide (5:3) stock solutions were determined at zero, two, four, six, 18, 24, 48 and 72 hr, respectively, in comparison to peak heights of the freshly prepared external standards at the same concentration level. We conclude that a 5:3 mixture of propofol and rocuronium bromide was stable up to 48 hr after mixing. Propofol concentration in the mixtures stored at ambient temperature showed degradation 72 hr after mixing. Propofol concentration in mixtures stored at 37°C showed degradation four hours after mixing. After Ethic’s Committee approval and patient written informed consent, 35 patients, ASA class I–II, undergoing elective surgery were included in this study. All patients received fentanyl 1 μg·kg–1 followed by three minutes of preoxygenation. Anesthesia was then induced with the propofol (2 mg·kg–1) rocuronium (0.6 mg·kg–1) mixture iv over 30 sec. All patients were intubated on the first attempt. The intubating conditions were evaluated using a score described by Viby-Mogensen.5 Intubating conditions at 60 sec were determined as excellent in 26 patients and good in nine patients. We have not determined any adverse effect of this mixture. We concluded that anesthesia induction with a propofol-rocuronium mixture provides excellent or good intubating conditions at 60 sec. It could be an effective and alternative technique for rapid induction of anesthesia.
Databáze: OpenAIRE
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