Methylation status of oestrogen receptor α-A: a predictor of prognosis in leukaemias
| Autor: | Xiao‑Bing Zhang, Wei‑Ling Fu, Xiao‑li Zhang, Jie Yao |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Biophysics Biology Methylation Biochemistry chemistry.chemical_compound Cell Line Tumor Humans Epigenetics Propidium iodide Receptor Molecular Biology Cell Proliferation Leukemia Cell growth Estrogen Receptor alpha Cell Biology Cell cycle Prognosis Molecular biology Symptomatic relief DNA demethylation chemistry Female |
| Zdroj: | Bioscience Reports. 30:217-222 |
| ISSN: | 1573-4935 0144-8463 |
| DOI: | 10.1042/bsr20090044 |
| Popis: | Many studies have shown that epigenetic regulation of ERs (oestrogen receptors) plays a key role in the pathogenesis of leukaemia. In the present study, it was found that the methylated status of ERalpha-A might serve as an epigenetic biomarker of leukaemias. In this study, the protein expression and cell apoptosis, cycle, proliferation and viability with and without 5-aza-dC (5-aza-2'-deoxycytidine) were evaluated with Western blotting, 3H-TdR (3H-thymidine) incorporation, propidium iodide staining and Trypan Blue staining respectively. The protein expression of ERalpha was significantly enhanced in all leukaemic cell lines using treatment with the DNA demethylation reagent 5-aza-dC. However, no obvious change in the protein expression of ERbeta takes place with 5-aza-dC. And with 5-aza-dC, cell apoptosis, cell cycle, cell proliferation and viability were all inhibited significantly. We also tracked 40 cases of leukaemias with ERalpha-A methylation (95%; 38 of 40) to observe the prognosis 1 year after chemotherapy treatment. The patients with ERalpha-A methylation have no obvious symptomatic relief; however, two patients without ERalpha-A methylation have obtained effective relief. This result suggested that ERalpha plays a significant role in leukaemogenesis, and the methylated status of ERalpha-A not only might serve as an epigenetic biomarker of leukaemias for diagnosis, but also has the potential to serve as a predictor of prognosis in leukaemias. |
| Databáze: | OpenAIRE |
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