Classical immunoproteomics: serological proteome analysis (SERPA) for antigen identification
| Autor: | Anna Ananchenko, Shannon S. Martin, Susan M. Twine, Lawrence A. Wolfraim, Kelly M. Fulton |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Biology Immunoproteomics Serology Tularemia live vaccine strain 03 medical and health sciences 0302 clinical medicine Immune system Antigen antigens medicine comparative immunoproteomics Francisella western blotting SERPA biomarkers medicine.disease biology.organism_classification immunoproteomics Tularemia vaccine tularemia 030104 developmental biology 030220 oncology & carcinogenesis Immunology Proteome serological proteome analysis immunoreactivity |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493995967 |
| Popis: | The study of the humoral immune response to infectious and chronic diseases is important for understanding the disease progression, identification of protective antigens, vaccine development, and discovery of biomarkers for early diagnosis. Proteomic approaches, including serological proteome analysis (SERPA), have been used to identify the repertoire of immunoreactive proteins in various diseases. In this chapter, we provide an outline of the SERPA approach, using the analysis of sera from mice vaccinated with a live attenuated tularemia vaccine as an example. Series: Methods in Molecular Biology |
| Databáze: | OpenAIRE |
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