No novel coronaviruses identified in a large collection of human nasopharyngeal specimens using family-wide CODEHOP-based primers
| Autor: | Kalina T. Zlateva, Marco C. Viveen, Margareta Ieven, Eric C. J. Claas, Alexander E. Gorbalenya, F.R. Leus, Frank E. J. Coenjaerts, Kelly M. Crusio, Willy J. M. Spaan, Christine Lammens |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Pcr assay RT-PCR Torovirus Respiratory tract infections medicine.disease_cause Medical microbiology Nasopharynx Virology medicine Humans Virus discovery DNA Primers Coronavirus biology Reverse Transcriptase Polymerase Chain Reaction Brief Report virus diseases General Medicine biology.organism_classification Reverse transcriptase CODEHOP Real-time polymerase chain reaction Healthy individuals Human medicine Real-time PCR |
| Zdroj: | Archives of virology Archives of Virology, 158(1), 251-255 Archives of Virology |
| ISSN: | 0304-8608 |
| Popis: | Novel viruses might be responsible for numerous disease cases with unknown etiology. In this study, we screened 1800 nasopharyngeal samples from adult outpatients with respiratory disease symptoms and healthy individuals. We employed a reverse transcription (RT)-PCR assay and CODEHOP-based primers (CT12-mCODEHOP) previously developed to recognize known and unknown corona- and toroviruses. The CT12-mCODEHOP assay detected 42.0 % (29/69) of samples positive for human coronaviruses (HCoV), including HCoV-229 (1/16), HCoV-NL63 (9/17), and HCoV-OC43 (19/36), and additionally HCoV-HKU1 (3), which was not targeted by the diagnostic real-time PCR assays. No other coronaviruses were identified in the analyzed samples. |
| Databáze: | OpenAIRE |
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