Properties of the A and A proteins of bacteriophage G4. The origin of G4 replicative-form DNA replication
| Autor: | Gerard A. van Arkel, Fons van Mansfeld, Cris Kuhlemeier, S.A. Langeveld, Peter Weisbeek |
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| Rok vydání: | 1981 |
| Předmět: |
DNA Replication
DNA clamp Base Sequence DNA polymerase II Ter protein DNA replication DNA Single-Stranded Biology Endonucleases Virus Replication Biochemistry Molecular biology Coliphages Single-stranded binding protein Molecular Weight Viral Proteins Replication factor C DNA Viral biology.protein Origin recognition complex Replication protein A |
| Zdroj: | European journal of biochemistry. 114(3) |
| ISSN: | 0014-2956 |
| Popis: | The A and A* proteins of the bacteriophage G4 have been purified. The proteins have been analysed for their enzymatic activities on single-stranded and double-stranded DNA. The A protein introduces a single-stranded break at a specific place in the G4 replicative form I DNA. This cleavage site has been localized between nucleotides 506 and 507 in the viral (+) strand. The A protein binds covalently to the 5′ end of the cleavage site. The A protein initiates the replication of the viral (+) DNA [Borrias, et al. (1979) Virology, 31, 288–298]; the cleavage site therefore identifies the origin of replication. The A* protein cleaves viral (+) strand DNA at many different sites and also binds covalently to the 5′ ends of the nick sites. The properties of both proteins strongly resemble the properties of the A and A* proteins of the related and much butter analysed phage oX 174. These results indicate that the G4 and oX 174 A and A* proteins have comparable functions and also that both phages initiate the replication of their replicative form DNA in a similar way. |
| Databáze: | OpenAIRE |
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