3-dimensional examination of the adult mouse subventricular zone reveals lineage-specific microdomains

Autor: Kazuaki Yoshikawa, Lutz Slomianka, Kasum Azim, Anahi Hurtado-Chong, Stefan Zweifel, Roberto Fiorelli, Olivier Raineteau
Přispěvatelé: University of Zurich, Raineteau, Olivier
Rok vydání: 2012
Předmět:
10017 Institute of Anatomy
lcsh:Medicine
Biochemistry
Mice
Lateral ventricles
0302 clinical medicine
Neural Stem Cells
Lateral Ventricles
Molecular Cell Biology
Basic Helix-Loop-Helix Transcription Factors
lcsh:Science
Brain Mapping
0303 health sciences
education.field_of_study
Multidisciplinary
Stem Cells
Gene Expression Regulation
Developmental
Neurochemistry
Anatomy
Neural stem cell
Cell biology
medicine.anatomical_structure
Cellular Types
Stem cell
Research Article
Neurogenesis
Green Fluorescent Proteins
Population
Subventricular zone
Mice
Transgenic
610 Medicine & health
1100 General Agricultural and Biological Sciences
Biology
03 medical and health sciences
Developmental Neuroscience
Neuroblast
1300 General Biochemistry
Genetics and Molecular Biology
medicine
Animals
Cell Lineage
Progenitor cell
education
030304 developmental biology
Progenitor
Homeodomain Proteins
1000 Multidisciplinary
lcsh:R
Mice
Inbred C57BL
570 Life sciences
biology
lcsh:Q
T-Box Domain Proteins
030217 neurology & neurosurgery
Developmental Biology
Neuroscience
Transcription Factors
Zdroj: PloS one
PLoS ONE, 7 (11)
PLoS ONE, Vol 7, Iss 11, p e49087 (2012)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0049087
Popis: Recent studies suggest that the subventricular zone (SVZ) of the lateral ventricle is populated by heterogeneous populations of stem and progenitor cells that, depending on their exact location, are biased to acquire specific neuronal fates. This newly described heterogeneity of SVZ stem and progenitor cells underlines the necessity to develop methods for the accurate quantification of SVZ stem and progenitor subpopulations. In this study, we provide 3-dimensional topographical maps of slow cycling “stem” cells and progenitors based on their unique cell cycle properties. These maps revealed that both cell populations are present throughout the lateral ventricle wall as well as in discrete regions of the dorsal wall. Immunodetection of transcription factors expressed in defined progenitor populations further reveals that divergent lineages have clear regional enrichments in the rostro-caudal as well as in the dorso-ventral span of the lateral ventricle. Thus, progenitors expressing Tbr2 and Dlx2 were confined to dorsal and dorso-lateral regions of the lateral ventricle, respectively, while Mash1+ progenitors were more homogeneously distributed. All cell populations were enriched in the rostral-most region of the lateral ventricle. This diversity and uneven distribution greatly impede the accurate quantification of SVZ progenitor populations. This is illustrated by measuring the coefficient of error of estimates obtained by using increasing section sampling interval. Based on our empirical data, we provide such estimates for all progenitor populations investigated in this study. These can be used in future studies as guidelines to judge if the precision obtained with a sampling scheme is sufficient to detect statistically significant differences between experimental groups if a biological effect is present. Altogether, our study underlines the need to consider the SVZ of the lateral ventricle as a complex 3D structure and define methods to accurately assess neural stem cells or progenitor diversity and population sizes in physiological or experimental paradigms.
PLoS ONE, 7 (11)
ISSN:1932-6203
Databáze: OpenAIRE
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