Biomimetic piezoelectric quartz crystal sensor with chloramphenicol-imprinted polymer sensing layer
| Autor: | Benilda S. Ebarvia, Isaiah E. Ubando, Fortunato Sevilla |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Chromatography Chloramphenicol Molecularly imprinted polymer Food Contamination Quartz Polymer Drug Residues Polymerization Analytical Chemistry Molecular Imprinting chemistry.chemical_compound Monomer Electricity Polymethacrylic Acids chemistry Methacrylic acid Biomimetics medicine Precipitation polymerization Molecular imprinting medicine.drug |
| Zdroj: | Talanta. 144:1260-1265 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2015.08.001 |
| Popis: | The measurement of banned antibiotic like chloramphenicol is significant for customer protection and safety. The presence of residual antibiotics in foods and food products of animal origin could pose as health hazards and affect food quality for global acceptance. In this study, the potential of a chloramphenicol sensor based on molecularly imprinted polymer (MIP) coupled with a piezoelectric quartz crystal was explored. The MIP was prepared by precipitation polymerization at 60 °C. Methacrylic acid was used as monomer, trimethylolpropane trimethacrylate (TRIM) as crosslinker, and chloramphenicol as the template. Template removal on the resulting polymer was done by extraction using methanol-acetic acid. Characterization of the MIP and NIP were conducted by spectroscopic and microscopic methods. These further supported the imprinting and rebinding process of chloramphenicol to the polymer matrix. The chloramphenicol sensor was devised by spin-coating onto one side of the 10 MHz AT-cut quartz crystal the MIP suspension in polyvinylchloride-tetrahydrofuran (6:2:1 w/w/v) solution. Optimization of sensor response was performed by varying the type of cross-linker, amount of MIP sensing layer, curing time, and pH. The sensor exhibited good sensitivity of about 73 Hz/log (conc., µg mL(-1)) and good repeatability (rsd |
| Databáze: | OpenAIRE |
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