Ovarian Grafts 10 Days after Xenotransplantation: Folliculogenesis and Recovery of Viable Oocytes
| Autor: | Luiz Sérgio de Almeida Camargo, Matheus Silvério Mattos, Marco Tulio Dias, Lucas Kraemer, João Henrique Moreira Viana, Paulo Henrique Almeida Campos-Junior, C. M. Assunção, Thalys Jair Melo Alves, Lucíola S. Barcelos, Brígida Gomes Almeida, Remo Castro Russo, Michele Munk |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Blastomeres Embryology Neutrophils Physiology medicine.medical_treatment Gene Expression lcsh:Medicine Mice SCID Mice White Blood Cells 0302 clinical medicine Ovarian Follicle Mice Inbred NOD Animal Cells Neoplasms Medicine and Health Sciences lcsh:Science 030219 obstetrics & reproductive medicine Multidisciplinary Hematology Body Fluids Ovaries Blood medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Oncology OVA Female Folliculogenesis Cellular Types Anatomy Perfusion Research Article medicine.medical_specialty Immune Cells Xenotransplantation Transplantation Heterologous Immunology Fertilization in Vitro Biology 03 medical and health sciences Internal medicine Genetics medicine Animals Blastocyst Ovarian follicle Blood Cells In vitro fertilisation Macrophages Ovary lcsh:R Reproductive System Biology and Life Sciences Peroxiredoxins Cell Biology Blastomere Transplantation Germ Cells 030104 developmental biology Endocrinology Gene Expression Regulation Oocytes Cattle Blastocysts lcsh:Q Developmental Biology |
| Zdroj: | PLoS ONE, Vol 11, Iss 6, p e0158109 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P |
| Databáze: | OpenAIRE |
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