Nucleotide sequence of cDNA encoding ZP3α, a sperm-binding glycoprotein from zona pellucida of pig oocyte
| Autor: | Jeffrey D. Harris, Anthony G. Sacco, Edward C. Yurewicz, Gregory K. Fontenot, David W. Hibler |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Signal peptide Swine Molecular Sequence Data Biophysics Receptors Cell Surface Sequence alignment Biology Zona Pellucida Glycoproteins Biochemistry Structural Biology Complementary DNA Genetics medicine Animals Humans Amino Acid Sequence Zona pellucida Peptide sequence Zona Pellucida Membrane Glycoproteins Base Sequence Sequence Homology Amino Acid cDNA library Egg Proteins Nucleic acid sequence Constitutive secretory pathway DNA Spermatozoa medicine.anatomical_structure Oocytes Female |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1174:211-214 |
| ISSN: | 0167-4781 |
| DOI: | 10.1016/0167-4781(93)90119-x |
| Popis: | We isolated a cDNA encoding the pig oocyte zona pellucida protein ZP3 alpha from a pig ovary lambda gt11 cDNA library. The 1699 bp cDNA contains a short 3' untranslated region characteristic of cDNAs encoding zona proteins. The deduced amino acid sequence for ZP3 alpha consists of 536 amino acid residues and shares 66% overall identity with a 55 kDa rabbit zona protein. Important features of the ZP3 alpha polypeptide include a predicted N-terminal signal sequence, twenty-two cysteine residues, an O-glycosylated domain and potential attachment sites for five N-linked sugar chains. A multibasic tetrapeptide occurs upstream of a predicted C-terminal transmembrane sequence; this suggests proteolytic processing of an integral membrane precursor within the constitutive secretory pathway. |
| Databáze: | OpenAIRE |
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