Identification, Molecular Characterization, and Occurrence of Two Bovine Hemoplasma Species in Swiss Cattle and Development of Real-Time TaqMan Quantitative PCR Assays for Diagnosis of Bovine Hemoplasma Infections
| Autor: | Regina Hofmann-Lehmann, Hans Lutz, Ute M. Dreher, Gabriela Knubben-Schweizer, Karl Nuss, Valentino Cattori, Ueli Braun, Marina L. Meli, Barbara Willi |
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| Přispěvatelé: | University of Zurich |
| Rok vydání: | 2010 |
| Předmět: |
10253 Department of Small Animals
Bacteremia Comorbidity Cattle Diseases medicine.disease_cause Polymerase Chain Reaction 2726 Microbiology (medical) Clinical Veterinary Microbiology law.invention 0403 veterinary science Mycoplasma law RNA Ribosomal 16S Cluster Analysis Phylogeny Polymerase chain reaction 0303 health sciences 630 Agriculture 04 agricultural and veterinary sciences 3. Good health Blood 11404 Department of Clinical Diagnostics and Services Switzerland DNA Bacterial Microbiology (medical) 040301 veterinary sciences Molecular Sequence Data Biology DNA Ribosomal 03 medical and health sciences medicine TaqMan Animals Mycoplasma Infections 030304 developmental biology Bacteriological Techniques Outbreak Sequence Analysis DNA biology.organism_classification medicine.disease Virology Molecular biology Mycoplasma haemofelis 10187 Department of Farm Animals Candidatus 570 Life sciences biology Cattle Anaplasmosis |
| Zdroj: | Journal of Clinical Microbiology. 48:3563-3568 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Concomitantly with an outbreak of fatal anaplasmosis in a cattle herd in Switzerland in 2002, we detected two bovine hemoplasma species in diseased animals: Mycoplasma wenyonii (formerly Eperythrozoon wenyonii ) and a second, novel bovine hemoplasma species later designated “ Candidatus Mycoplasma haemobos” (synonym, “ Candidatus Mycoplasma haemobovis”). The second species was characterized by a shorter 16S rRNA gene. The aims of the present study were to provide a detailed molecular characterization of this species, to develop specific quantitative real-time PCR assays for the two bovine hemoplasma species, and to apply these assays in order to evaluate the prevalence and clinical significance of the hemoplasmas. Sequencing of the near-complete 16S rRNA gene of the second hemoplasma revealed that it was 94% identical to that of Mycoplasma haemofelis , an anemia-inducing feline hemoplasma species, but less than 85% identical to that of the bovine hemoplasma M. wenyonii . Using the newly developed assays, a total of 159 animals from the anaplasmosis outbreak were reexamined. In addition, we tested 57 clinically ill and 61 healthy Swiss cattle, as well as 47 calves. Both hemoplasmas were highly prevalent in adult cattle but occurred rarely in calves. Animals from the herd with the fatal anemia outbreak were more frequently infected with M. wenyonii and exhibited higher M. wenyonii blood loads than animals with unrelated diseases and healthy animals. Coinfections may increase the pathogenicity and clinical significance of bovine hemoplasmosis. |
| Databáze: | OpenAIRE |
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