Extracts of pine bark (Pinus sylvestris) inhibit Cryptosporidium parvum growth in cell culture
Autor: | Håvard Steinshamn, Stig Milan Thamsborg, Inga Marie Aasen, Ian David Woolsey, Kristin Sørheim, Berit Marie Blomstrand, Karl-Christian Mahnert, Øivind Øines, Heidi L. Enemark, Spiridoula Athanasiadou |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Cell Culture Techniques Treatment and Prophylaxis - Original Paper Health and welfare complex mixtures Microbiology 03 medical and health sciences chemistry.chemical_compound Cell Line Tumor parasitic diseases Parasite hosting Humans Condensed tannins Cryptosporidium parvum General Veterinary biology Inoculation Plant Extracts Scots pine Cryptosporidium Pinus sylvestris General Medicine 030108 mycology & parasitology biology.organism_classification 030104 developmental biology Infectious Diseases chemistry Proanthocyanidin Insect Science visual_art Pine bark visual_art.visual_art_medium Plant Bark Parasitology Bark Growth inhibition |
Zdroj: | Parasitology Research 2919–2927 Blomstrand, B M, Enemark, H L, Øines, Ø, Steinshamn, H, Aasen, I M, Mahnert, K C, Sørheim, K M, Athanasiadou, S, Thamsborg, S M & Woolsey, I D 2021, ' Extracts of pine bark ( Pinus sylvestris ) inhibit Cryptosporidium parvum growth in cell culture ', Parasitology Research, vol. 120, pp. 2919–2927 . https://doi.org/10.1007/s00436-021-07220-w |
Popis: | The widespread apicomplexan parasite Cryptosporidium parvum is responsible for severe gastrointestinal disease in humans and animals. The treatment options are limited, and the efficacy of available drugs is low. Bark contains condensed tannins (CT), which are bioactive compounds previously shown to inhibit parasite development. Here, we examined the anti-cryptosporidial properties of bark extract of Scots pine (Pinus sylvestris) against C. parvum by means of an in vitro growth inhibition test. We hypothesised that bark extracts would have dose-dependent inhibitory effects on the development of C. parvum in cell culture.Bark extracts from Scots pine extracted with acetone, methanol, and water as solvents were investigated using human colorectal adenocarcinoma cells infected with C. parvum. Oocysts were inoculated onto the cell monolayer and bark extract was added at seven different concentrations. Parasite growth inhibition was quantified by qPCR.The acetone and methanol extracts demonstrated a sigmoid dose-dependent inhibition of C. parvum. The IC50 values were 244.6 and 279.1 µg dry matter extract/mL, and 25.4 and 24.1 µg CT/mL, for acetone and methanol extracts, respectively. The IC50 for both extracts were similar, both with regard to the dry matter concentration of each extract and to CT concentrations.Given the limited treatment options available for Cryptosporidium spp., the evidence generated in our study encourages further investigation into the in vitro and in vivo effects of pine bark extracts against C. parvum. |
Databáze: | OpenAIRE |
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