Antiherpetic Properties of Acyclovir 5′-Hydrogenphosphonate and the Mutation Analysis of Herpes Virus Resistant Strains
| Autor: | Maxim V. Yasko, A. A. Gus’kova, Inna L. Karpenko, A. N. Korovina, Mikhail Skoblov, Marina K. Kukhanova, Yuri S. Skoblov, Valeria L. Andronova, George A. Galegov |
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| Rok vydání: | 2009 |
| Předmět: |
viruses
Molecular Sequence Data Population Acyclovir Biology medicine.disease_cause Antiviral Agents Thymidine Kinase Biochemistry Microbiology Mice Herpes virus Chlorocebus aethiops Drug Resistance Viral Drug Discovery medicine Animals Amino Acid Sequence Amino acid residue skin and connective tissue diseases education Vero Cells Herpesviridae Pharmacology Mice Inbred BALB C education.field_of_study Strain (chemistry) Organic Chemistry virus diseases Herpesviridae Infections Virology Herpes simplex virus Thymidine kinase Mutation Mutation testing Vero cell Molecular Medicine Sequence Alignment |
| Zdroj: | Chemical Biology & Drug Design. 74:382-389 |
| ISSN: | 1747-0285 1747-0277 |
| Popis: | In this study, we continued to study antiherpetic properties of acyclovir 5¢-hydrogenphosphonate (Hp-ACV) in cell cultures and animal models. HpACV was shown to inhibit the development of herpetic infection in mice induced by the HSV-1 ⁄ L2 strain. The compound suppressed replication of both ACV-sensitive HSV-1 ⁄ L2 and ACV-resistant HSV-1 ⁄ L2 ⁄ R strains in Vero cell culture. Viral population resistant to Hp-ACV (HSV-1 ⁄ L2 ⁄ RHp-ACV) was developed much slower than ACV-resistant population. The analysis of Hp-ACV-resistant clones isolated from the HSV-1 ⁄ L2 ⁄ RHp-ACV population demonstrated their partial cross-resistance to ACV. The mutations determining the resistance of HSV-1 clones to Hp-ACV were partly overlapped with mutations defining ACV resistance but did not always coincide. HSV-1 ⁄ L2 ⁄ RHp-ACV herpes virus thymidine kinase is shortened from the C-terminus by 100 amino acid residues in length. |
| Databáze: | OpenAIRE |
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