Detection of border disease virus (BDV) genotype 3 in Italian goat herds
| Autor: | P. Caroggio, Alessandro Dondo, Loretta Masoero, Pier Luigi Acutis, V. Tarello, Alfonso Rosamilia, E. Gobbi, Claudio Caruso, Simone Peletto, Carla Grattarola |
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| Rok vydání: | 2014 |
| Předmět: |
Veterinary medicine
Genotype viruses animal diseases Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Virus Border disease virus Antigen medicine Animals Mesenteric lymph nodes Phylogeny Goat Diseases Border Disease General Veterinary biology Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis RNA Goats Pestivirus virus diseases biology.organism_classification Virology medicine.anatomical_structure Italy Herd biology.protein RNA Viral Animal Science and Zoology Antibody 5' Untranslated Regions |
| Zdroj: | The Veterinary Journal. 199:446-450 |
| ISSN: | 1090-0233 |
| DOI: | 10.1016/j.tvjl.2013.12.006 |
| Popis: | In January 2011, cases of abortion, stillbirth and weak live kids were reported in two goat herds in northern Italy. Samples from 18 kids found dead, 12 fetuses, and two stillborn kids were analyzed for pestivirus antigen using an ELISA kit and a border disease virus (BDV)-specific RT-PCR. Positive results were obtained in six kids and one fetus. Phylogenetic analysis based on 225 bp of the 5'UTR fragment of the BDV genome from positive samples showed that the goats were infected with BDV genotype 3. Serum and blood samples collected from all animals in both herds were analyzed using competitive ELISA to detect p80 antibodies and RT-PCR to detect viraemia. Pestivirus antibodies were detected in 61/67 goats in herd A and in 38/169 in herd B. A persistently infected (PI) goat was found in herd A. The PI animal was submitted to the laboratory for BDV diagnosis with Ag-ELISA, viral isolation, and nested RT-PCR on tissue samples from the spleen, kidney, brain, liver, lung, ileocaecal valve, mesenteric lymph nodes, and skin. All of the tests were positive for BDV in each of the tissues analyzed. The BDV sequence of the PI was identical to BDV sequences found in other positive animals. This is the first description of a BDV PI goat and the first evidence of BDV genotype 3 circulation in Italy. The study raises questions about the real impact this virus has on breeding goats. |
| Databáze: | OpenAIRE |
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