Mosquitocidal activities of Chenopodium botrys whole plant n-hexane extract against Culex quinquefasciatus
| Autor: | I. Ilahi, A. M. Yousafzai, M. Attaullah, T. U. Haq, A. Rahim, W. Khan, A. A. Khan, S. Ullah, T. Jan, M. M. Khan, G. Rahim, N. Zaman |
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| Rok vydání: | 2023 |
| Předmět: |
Insecticides
column chromatography QH301-705.5 Science column fractions adulticidal Biology Chenopodium chemistry.chemical_compound Column chromatography parasitic diseases Animals Hexanes Bioassay pupicidal Biology (General) Larva Traditional medicine Plant Extracts fungi Botany Chenopodium botrys biology.organism_classification Disease control Culex quinquefasciatus Plant Leaves Hexane Culex QL1-991 chemistry QK1-989 Instar larvicidal General Agricultural and Biological Sciences Zoology |
| Zdroj: | Brazilian Journal of Biology v.83 2023 Brazilian Journal of Biology Instituto Internacional de Ecologia (IIE) instacron:IIE Brazilian Journal of Biology, Vol 83 (2021) |
| ISSN: | 1678-4375 1519-6984 |
| DOI: | 10.1590/1519-6984.240842 |
| Popis: | This research aimed to investigate various mosquitocidal activities of Chenopodium botrys whole- plant n-hexane extract against Culex quinquefasciatus. The extract showed remarkable larvicidal, pupicidal, adulticidal, oviposition deterrent and adult emergence inhibitory activities against Cx. quinquefasciatus. During the larvicidal and pupicidal activities, the 24-hour lethal concentration (LC50) of extract against 2nd instar larvae, 4th instar larvae and pupae were 324.6, 495.6 and 950.8 ppm, respectively. During the CDC (Centers for Disease Control and Prevention) bottle bioassay for adulticidal activity, the median knockdown times (KDT50) at 1.25% concentration was 123.4 minutes. During the filter paper impregnation bioassay for adulticidal activity, the KDT50 value at 0.138 mg/cm2 concentration was 48.6 minutes. The extract was fractionated into 14 fractions through silica gel column chromatography which were then combined into six fractions on the basis of similar retention factor (Rf) value. These fractions were screened for adulticidal activity by applying CDC bottle bioassay. The fraction obtained through 60:40 to 50:50% n-hexanes-chloroform mobile phase with 0.5 Rf value showed 100% adulticidal activity at 0.2% concentration. During oviposition deterrent activity, the highest concentration (1000 ppm) showed 71.3 ± 4.4% effective repellence and 0.6 ± 0.1 oviposition activity index. During adult emergence inhibition activity, the median emergence inhibition (EI50) value was 312.3 ppm. From the outcome of the present investigation, it is concluded that the n-hexane extract of C. botrys whole- plant possesses strong larvicidal, pupicidal, adulticidal, oviposition deterrent and adult emergence inhibitory activities against Cx. quinquefasciatus. |
| Databáze: | OpenAIRE |
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