Wogonin alleviates liver injury in sepsis through Nrf2‐mediated NF‐κB signalling suppression
Autor: | Kunwei Niu, Cheng-Li Liu, Weinan Guo, Zhi-Nan Chen, Jia-Jia Zhang, Yi-Zhou Tan, Jimin Dai, Kaishan Tao, Jing-Yao Dai, Xiang-Min Yang |
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Rok vydání: | 2021 |
Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Lipopolysaccharide NF-E2-Related Factor 2 SOD1 SOD2 wogonin Pharmacology medicine.disease_cause Nrf2 sepsis Sepsis Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Wogonin medicine Animals Liver injury biology business.industry NF-kappa B Original Articles Cell Biology Liver Failure Acute biology.organism_classification medicine.disease Mice Inbred C57BL Disease Models Animal Oxidative Stress 030104 developmental biology chemistry 030220 oncology & carcinogenesis Flavanones Molecular Medicine Scutellaria baicalensis Original Article business Oxidative stress liver injury Signal Transduction |
Zdroj: | Journal of Cellular and Molecular Medicine |
ISSN: | 1582-4934 1582-1838 |
Popis: | Sepsis is a life‐threatening organ dysfunction syndrome, and liver is a susceptible target organ in sepsis, because the activation of inflammatory pathways contributes to septic liver injury. Oxidative stress has been documented to participate in septic liver injury, because it not only directly induces oxidative genotoxicity, but also exacerbates inflammatory pathways to potentiate damage of liver. Therefore, to ameliorate oxidative stress is promising for protecting liver in sepsis. Wogonin is the compound extracted from the medicinal plant Scutellaria baicalensis Geogi and was found to exert therapeutic effects in multiple inflammatory diseases via alleviation of oxidative stress. However, whether wogonin is able to mitigate septic liver injury remains unknown. Herein, we firstly proved that wogonin treatment could improve survival of mice with lipopolysaccharide (LPS)‐ or caecal ligation and puncture (CLP)‐induced sepsis, together with restoration of reduced body temperature and respiratory rate, and suppression of several pro‐inflammatory cytokines in circulation. Then, we found that wogonin effectively alleviated liver injury via potentiation of the anti‐oxidative capacity. To be specific, wogonin activated Nrf2 thereby promoting expressions of anti‐oxidative enzymes including NQO‐1, GST, HO‐1, SOD1 and SOD2 in hepatocytes. Moreover, wogonin‐induced Nrf2 activation could suppress NF‐κB‐regulated up‐regulation of pro‐inflammatory cytokines. Ultimately, we provided in vivo evidence that wogonin activated Nrf2 signalling, potentiated anti‐oxidative enzymes and inhibited NF‐κB‐regulated pro‐inflammatory signalling. Taken together, this study demonstrates that wogonin can be the potential therapeutic agent for alleviating liver injury in sepsis by simultaneously ameliorating oxidative stress and inflammatory response through the activation of Nrf2. |
Databáze: | OpenAIRE |
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