Antibiotics promoting oxidative stress inhibit formation of Escherichia coli biofilm via indole signalling
| Autor: | Daria Leszczyńska, Dorota Kuczyńska-Wiśnik, Ewelina Matuszewska, Beata Furmanek-Blaszk, Ewa Laskowska, Paweł Szczepaniak, Alicja Grudowska |
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| Rok vydání: | 2010 |
| Předmět: |
Indoles
medicine.disease_cause Microbiology chemistry.chemical_compound Escherichia coli medicine Dimethyl Sulfoxide Hydrogen peroxide Molecular Biology chemistry.chemical_classification Indole test Reactive oxygen species biology Gene Expression Profiling Tryptophanase Biofilm Free Radical Scavengers General Medicine biology.organism_classification Anti-Bacterial Agents Oxidative Stress chemistry Biochemistry Biofilms Reactive Oxygen Species Oxidative stress Bacteria Signal Transduction |
| Zdroj: | Research in Microbiology. 161:847-853 |
| ISSN: | 0923-2508 |
| Popis: | Recent studies have revealed that antibiotics can promote the formation of reactive oxygen species which contribute to cell death. In this study, we report that five different antibiotics known to stimulate production of reactive oxygen species inhibited growth of Escherichia coli biofilm. We demonstrated that supression of biofilm formation was mainly a consequence of the increase in the extracellular concentration of indole, a signal molecule which suppresses growth of bacterial biofilm. Indole production was enhanced under antibiotic-mediated oxidative stress due to overexpression of tryptophanase (TnaA), which catalyzes synthesis of indole. We found that DMSO (dimethyl sulfoxide), a hydrogen peroxide scavenger, or the lack of trypthophanase, which catalyzes production of indole, partly restored formation of E. coli biofilm in the presence of antibiotics. In conclusion, these findings confirmed that antibiotics which promote formation of ROS (reactive oxygen species) can inhibit development of E. coli biofilm in an indole-dependent process. |
| Databáze: | OpenAIRE |
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