Characterization of a temperate phage hosted by Alcaligenes eutrophus strain A5
| Autor: | Michel Faelen, M Geuskens, Max Mergeay, Ariane Toussaint, Christophe Merlin |
|---|---|
| Přispěvatelé: | Université libre de Bruxelles (ULB), Laboratoire de Biochimie des Microorganismes, Université Joseph Fourier - Grenoble 1 (UJF), Belgian Center for Nuclear Energy |
| Rok vydání: | 1993 |
| Předmět: |
viruses
Mitomycin Restriction Mapping In Vitro Techniques Microbiology Bacteriophage 03 medical and health sciences chemistry.chemical_compound Bacteriophages Alcaligenes Molecular Biology ComputingMilieux_MISCELLANEOUS Prophage 030304 developmental biology 0303 health sciences biology Strain (chemistry) 030306 microbiology General Medicine Provirus biology.organism_classification Temperateness Restriction enzyme Microscopy Electron chemistry [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology DNA Viral Electrophoresis Polyacrylamide Gel Bacteria DNA |
| Zdroj: | Research in Microbiology Research in Microbiology, Elsevier, 1993, 144 (8), pp.627-631. ⟨10.1016/0923-2508(93)90065-A⟩ |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/0923-2508(93)90065-A⟩ |
| Popis: | Nineteen strains of Alcaligenes eutrophus were tested for the presence of prophages. One strain that lysed upon mitomycin C treatment produced a phage which could not form plaques on any of the strains available. DNA extracted from partially purified phage lysates was digested with various restriction enzymes which showed that the 42 kb long viral double-stranded DNA circularizes by means of cohesive ends. To our knowledge, this is the first description of a phage for the genus Alcaligenes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|