Role of GM-CSF in a mouse model of experimental autoimmune prostatitis
| Autor: | Jianfeng Cui, Qinggang Liu, Benkang Shi, Yaxiao Liu, Hongda Zhao, Yan Li, Kejia Zhu, Changkuo Zhou, Zonglong Wu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pelvic pain syndrome Male Physiology education Prostatitis Gene Expression Granulocyte Pelvic Pain Autoimmune Diseases 03 medical and health sciences Mice 0302 clinical medicine Semen Ganglia Spinal medicine Macrophage Animals Humans RNA Messenger Mice Knockout business.industry Prostate Granulocyte-Macrophage Colony-Stimulating Factor medicine.disease Disease Models Animal 030104 developmental biology medicine.anatomical_structure Granulocyte macrophage colony-stimulating factor Receptors Granulocyte-Macrophage Colony-Stimulating Factor Immunology Etiology Chronic Pain business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | American journal of physiology. Renal physiology. 317(7) |
| ISSN: | 1522-1466 |
| Popis: | The etiology of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is still unknown. Granulocyte macrophage colony-stimulating factor (GM-CSF) has been shown to play an important role in the development of autoimmune and inflammatory diseases. Here, we investigated the expression and function of GM-CSF in patients with CP/CPPS and in a mouse model of experimental autoimmune prostatitis (EAP). GM-CSF mRNA levels were detected in expressed prostatic secretions samples from patients with CP/CPPS and in prostate tissue from a mouse model of EAP. The expression of GM-CSF receptor in mouse prostate and dorsal root ganglia were determined using PCR and immunohistochemistry. Behavioral testing and inflammation scoring were performed to evaluate the role of GM-CSF in disease development and symptom severity of EAP using GM-CSF knockout mice. mRNA levels of putative nociceptive and inflammatory markers were measured in the prostate after the induction of EAP. Elevated GM-CSF mRNA levels were observed in expressed prostatic secretions samples from patients with CP/CPPS compared with healthy volunteers. GM-CSF mRNA was also significantly increased in prostate tissue of the EAP mice model. The expression of GM-CSF receptors was confirmed in mouse prostate and dorsal root ganglia. GM-CSF knockout mice showed fewer Infiltrating leukocytes and pain symptoms after the induction of EAP. Deletion of GM-CSF significantly diminished EAP-induced increases of chemokine (C-C motif) ligand 2, chemokine (C-C motif) ligand 3, and nerve growth factor mRNA expression. The results indicated that GM-CSF plays a functional role in the pathogenesis of EAP. GM-CSF may function as a signaling mediator for both inflammation and pain transduction in CP/CPPS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|