Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
| Autor: | J. M. Sandra Healy, James A. H. Murray, Catherine Riou-Khamlichi, Annie Jacqmard, Nigel J. Kilby, Simon Scofield, Walter Dewitte |
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| Rok vydání: | 2003 |
| Předmět: |
Cellular differentiation
Meristem Arabidopsis Cell Cycle Proteins Plant Science Biology Palisade cell Gene Expression Regulation Enzymologic Mitotic cell cycle Gene Expression Regulation Plant Cyclins Endoreduplication Cyclin D3 Flowering Tops Epidermis (botany) Cell growth Arabidopsis Proteins Cell Cycle Gene Expression Regulation Developmental Cell Differentiation Cell Biology Cell cycle Cyclin-Dependent Kinases Cell biology Cell Division Plant Shoots Transcription Factors Research Article |
| Zdroj: | The Plant cell. 15(1) |
| ISSN: | 1040-4651 |
| Popis: | CYCD3;1 expression in Arabidopsis is associated with proliferating tissues such as meristems and developing leaves but not with differentiated tissues. Constitutive overexpression of CYCD3;1 increases CYCD3;1-associated kinase activity and reduces the proportion of cells in the G1-phase of the cell cycle. Moreover, CYCD3;1 overexpression leads to striking alterations in development. Leaf architecture in overexpressing plants is altered radically, with a failure to develop distinct spongy and palisade mesophyll layers. Associated with this, we observe hyperproliferation of leaf cells; in particular, the epidermis consists of large numbers of small, incompletely differentiated polygonal cells. Endoreduplication, a marker for differentiated cells that have exited from the mitotic cell cycle, is inhibited strongly in CYCD3;1-overexpressing plants. Transcript analysis reveals an activation of putative compensatory mechanisms upon CYCD3;1 overexpression or subsequent cell cycle activation. These results demonstrate that cell cycle exit in the G1-phase is required for normal cellular differentiation processes during plant development and suggest a critical role for CYCD3 in the switch from cell proliferation to the final stages of differentiation. |
| Databáze: | OpenAIRE |
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