Association of homeobox B13 (HOXB13) gene variants with prostate cancer risk in an Iranian population
| Autor: | Behzad Narouie, Mahsa Rastegar Moghadam, Azadeh Shojaei, Mehdi Sotoudeh, Raheleh Roudi, Hosein Nemati |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Tumor initiation Iran urologic and male genital diseases medicine.disease_cause 03 medical and health sciences Prostate cancer Exon 0302 clinical medicine Internal medicine medicine Clinical significance Variant Gene Mutation Benign prostatic hyperplasia business.industry HOXB13 General Medicine Hyperplasia medicine.disease genomic DNA 030104 developmental biology 030220 oncology & carcinogenesis Original Article business |
| Zdroj: | Medical Journal of the Islamic Republic of Iran |
| ISSN: | 2251-6840 1016-1430 |
| DOI: | 10.14196/mjiri.32.97 |
| Popis: | Background: Prostate cancer is a complex condition in which both genetic and environmental factors concomitantly contribute to the tumor initiation and progression. Recently, HOXB13 has been proposed as a susceptibility gene for prostate cancer. Objective: The present study was conducted to determine the existence of potential variations in HOXB13 gene in Iranian men with prostate cancer (PCa) compared to benign prostatic hyperplasia (BPH) cases. Methods: HOXB13 genetic status was screened in 51 samples, including 21 blood and tissue of PCa cases, and compared to 30 cases affected by BPH using PCR/sequencing. Then, the existence of potential association was investigated between genomic DNA alterations in blood and tissue PCa specimens. Results: Analysis of BPH tissues showed single nucleotide variations c.366C > T (rs) or c.513T > C (rs9900627) in exon 1, but not in exon 2. Evaluation of PCa tissues revealed 2 cases with both synonymous c.366C > T and c.513T > C variants and 2 cases with the synonymous c.366C > T variant in exon 1. The variants c.366C > T and c.513T > C, simultaneously or separately, were found in blood samples of PCa patients. The novel variant c.127A > G in exon 2 was detected in 1 PCa blood sample. Our analysis indicated a significant reciprocal correlation between HOXB13 mutation in the tissue and blood samples of PCa cases (p= 0.02). Conclusion: The variants in exon 2 of HOXB13 may influence the risk of prostate cancer. Also, evaluation of HOXB13 mutation may be considered as a novel marker for screening PCa. Further investigations are warranted to evaluate the clinical significance of HOXB13 in Iranian population. |
| Databáze: | OpenAIRE |
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