Transcription destabilizes centromere function
| Autor: | Masayuki Seki, Yu Nakabayashi |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Models
Molecular Saccharomyces cerevisiae Proteins Transcription Genetic Chromosomal Proteins Non-Histone Protein Conformation Centromere Biophysics Mitosis Saccharomyces cerevisiae Biology Microtubules Biochemistry Histones Chromosome segregation Transcription (biology) Chromosome Segregation Gene Expression Regulation Fungal Protein Isoforms Nucleosome Kinetochores Molecular Biology Histone binding Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Chromatin binding Cell Biology Nucleosomes Cell biology DNA-Binding Proteins Histone biology.protein Centromere Protein A Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 586:150-156 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2021.11.077 |
| Popis: | Bi-oriented attachment of microtubules to the centromere is a pre-requisite for faithful chromosome segregation during mitosis. Budding yeast have point centromeres containing the cis-element proteins CDE-I, -II, and -III, which interact with trans-acting factors such as Cbf1, Cse4, and Ndc10. Our previous genetic screens, using a comprehensive library of histone point mutants, revealed that the TBS-I, -II, and -III regions of nucleosomes are required for faithful chromosome segregation. In TBS-III deficient cells, peri-centromeric nucleosomes containing the H2A.Z homolog Htz1 are lacking, however, it is unclear why chromosome segregation is defective in these cells. Here, we show that, in cells lacking TBS-III, both chromatin binding at the centromere and the total amount of some of the centromere proteins are reduced, and transcription through the centromere is up-regulated during M-phase. Moreover, the chromatin binding of Cse4, Mif2, Cbf1, Ndc10, and Scm3 was reduced upon ectopic transcription through the centromere in wild-type cells. These results suggest that transcription through the centromere displaces key centromere proteins and, consequently, destabilizes the interaction between centromeres and microtubules, leading to defective chromosome segregation. The identification of new roles for histone binding residues in TBS-III will shed new light on nucleosome function during chromosome segregation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect