Classification of propionic acid bacteria and approaches to applied genetics
| Autor: | Susanne Miescher, Melanie P. Stierli, Leo Meile, Gottfried Dasen, Michael Teuber |
|---|---|
| Přispěvatelé: | Revues Inra, Import |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Genetics
0303 health sciences Phylogenetic tree biology 030306 microbiology [SDV.IDA] Life Sciences [q-bio]/Food engineering biology.organism_classification 16S ribosomal RNA Microbiology law.invention 03 medical and health sciences [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition Plasmid Bacteriocin Phylogenetics law [SDV.IDA]Life Sciences [q-bio]/Food engineering Ribosomal DNA [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Bacteria Polymerase chain reaction ComputingMilieux_MISCELLANEOUS 030304 developmental biology Food Science |
| Zdroj: | Le Lait Le Lait, INRA Editions, 1999, 79 (1), pp.71-78 |
| Popis: | Strains of the genus Propionibacterium, which were recently c1assified within the new c1ass Aetinobaeteria, are grouped into cutaneous (medical) or dairy (c1assical) propionic acid bacteria. 16S rDNA sequences of ail Propionibaeterium type strains and sorne new isolates were completed. A phylogenetic tree was constructed and current trees reexamined. The c1assical species P. thoenii, P. jensenii and P. acidipropionici were c1ustered in a group distinct from both the closely related elus- ters of P.freudenreiehii and P. eyclohexanieum. Within the cutaneous species, three groups were elus- tered: P. granulosum forms a distinct c1uster between the c1assical groups and a group containing P. avidum, P. aenes and P. propionicum, whereas P. lymphophilum is placed more distantly from any group in this tree. In order to differentiate Propionibaeterium isolates from other food-isolates a rapid multiplex-PCR (MPCR) method based on 16S rRNA-targeted oligonucleotides and particularly on a 16S rDNA-motif which tumed out to he specifie for the genus Propionibaeterium was developed. The MPCR-amplification could be performed within 1 d and reached detection limits of 103 colony forming units or 35 pg of DNA. The MPCR-method was used in various Propionibaeterium screen- ing studies. In the course of screening antifungal and antibacterial activities of propionic acid bacteria, a Propionibaeterium jensenii strain containing a cryptic 7-kb plasmid was identified. This strain produced the bacteriocin Propionicin SM 1. Purification of Propionicin SM 1 and cloning of its genetic locus is currently under way. © Inra/Elsevier, Paris. propionibacteria / classification / PCR-identification /168 rDNA / genetic |
| Databáze: | OpenAIRE |
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