Mouse Paneth cell antimicrobial function is independent of Nod2
| Autor: | Andrew M. White, Anthony A. Fodor, Susan J. Henning, Emily Grossniklaus, Ajay S. Gulati, Michael J. Shanahan, R. Balfour Sartor, Ian M. Carroll, Roshonda Barner, Richard J. von Furstenberg |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Paneth Cells
alpha-Defensins Transcription Genetic Antimicrobial peptides Nod2 Signaling Adaptor Protein Pancreatitis-Associated Proteins Microbial Sensitivity Tests Biology Microbiology Flow cytometry Defensins 03 medical and health sciences Feces 0302 clinical medicine Antigens Neoplasm Ileum NOD2 medicine Biomarkers Tumor Escherichia coli Animals Lectins C-Type RNA Messenger Protein Precursors Polyacrylamide gel electrophoresis Gene 030304 developmental biology Mice Knockout 0303 health sciences medicine.diagnostic_test Gastroenterology Salmonella enterica Ribonuclease Pancreatic Antimicrobial Intestinal epithelium digestive system diseases Mice Inbred C57BL medicine.anatomical_structure Paneth cell 030211 gastroenterology & hepatology Muramidase Peptides |
| DOI: | 10.17615/67gk-ya25 |
| Popis: | Objective Although polymorphisms of the NOD2 gene predispose to the development of ileal Crohn9s disease, the precise mechanisms of this increased susceptibility remain unclear. Previous work has shown that transcript expression of the Paneth cell (PC) antimicrobial peptides (AMPs) α-defensin 4 and α-defensin-related sequence 10 are selectively decreased in Nod2 −/− mice. However, the specific mouse background used in this previous study is unclear. In light of recent evidence suggesting that mouse strain strongly influences PC antimicrobial activity, we sought to characterise PC AMP function in commercially available Nod2 −/− mice on a C57BL/6 (B6) background. Specifically, we hypothesised that Nod2 −/− B6 mice would display reduced AMP expression and activity. Design Wild-type (WT) and Nod2 −/− B6 ileal AMP expression was assessed via real-time PCR, acid urea polyacrylamide gel electrophoresis and mass spectrometry. PCs were enumerated using flow cytometry. Functionally, α-defensin bactericidal activity was evaluated using a gel-overlay antimicrobial assay. Faecal microbial composition was determined using 454-sequencing of the bacterial 16S gene in cohoused WT and Nod2 −/− littermates. Results WT and Nod2 −/− B6 mice displayed similar PC AMP expression patterns, equivalent α-defensin profiles, and identical antimicrobial activity against commensal and pathogenic bacterial strains. Furthermore, minimal differences in gut microbial composition were detected between the two cohoused, littermate mouse groups. Conclusions Our data reveal that Nod2 does not directly regulate PC antimicrobial activity in B6 mice. Moreover, we demonstrate that previously reported Nod2-dependent influences on gut microbial composition may be overcome by environmental factors, such as cohousing with WT littermates. |
| Databáze: | OpenAIRE |
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