Implementation of comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for the simultaneous determination of halogenated contaminants and polycyclic aromatic hydrocarbons in fish
| Autor: | Tomas Cajka, Jana Pulkrabova, Lucie Drabova, Jana Hajslova, Kamila Kalachova |
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| Rok vydání: | 2012 |
| Předmět: |
Chrysene
Time Factors Resolution (mass spectrometry) 02 engineering and technology 01 natural sciences Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Polybrominated diphenyl ethers Animals Polycyclic Aromatic Hydrocarbons Fluoranthene Anthracene Chromatography Hydrocarbons Halogenated 010401 analytical chemistry Fishes 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Pyrene Environmental Pollutants Gas chromatography Gas chromatography–mass spectrometry 0210 nano-technology Food Analysis |
| Zdroj: | Analytical and Bioanalytical Chemistry |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-012-6095-3 |
| Popis: | In the presented study, comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC × GC-TOFMS) was shown to be a powerful tool for the simultaneous determination of various groups of contaminants including 18 polychlorinated biphenyls (PCBs), seven polybrominated diphenyl ethers (PBDEs), and 16 polycyclic aromatic hydrocarbons (PAHs). Since different groups of analytes (traditionally analyzed separately) were included into one instrumental method, significant time savings were achieved. Following the development of an integrated sample preparation procedure for an effective and rapid isolation of several groups of contaminants from fish tissue, the GC × GC-TOFMS instrumental method was optimized to obtain the best chromatographic resolution and low quantification limits (LOQs) of all target analytes in a complex mixture. Using large-volume programmable temperature vaporization, the following LOQs were achieved-PCBs, 0.01-0.25 μg/kg; PBDEs, 0.025-5 μg/kg; PAHs 0.025-0.5 μg/kg. Furthermore, several capillary column combinations (BPX5, BPX50, and Rxi-17Sil-ms in the first dimension and BPX5, BPX50, Rt-LC35, and HT8 in the second dimension) were tested during the experiments, and the optimal separation of all target analytes even of critical groups of PAHs (group (a): benz[a]anthracene, cyclopenta[cd]pyrene and chrysene; group (b): benzo[b]fluoranthene, benzo[j]fluoranthene and benzo[k]fluoranthene; group (c): dibenz[ah]anthracene, indeno[1,2,3-cd]pyrene and benzo[ghi]perylene) was observed on BPX5 × BPX50 column setup. Moreover, since the determination of target analytes was performed using TOFMS detector, further identification of other non-target compounds in real life samples was also feasible. |
| Databáze: | OpenAIRE |
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