Endothelium nitric oxide-independent vasorelaxant effects of the aqueous extract from Stephania abyssinica on the isolated rat thoracic aorta
Autor: | Télesphore Benoît Nguelefack, Paulin Nyadjeu, Sylvie Léa Wansi, Chamberlin Fodem, Pierre Watcho, Albert Kamanyi, Elvine Pami Nguelefack-Mbuyo |
---|---|
Rok vydání: | 2014 |
Předmět: |
Male
Contraction (grammar) Endothelium Stereochemistry Vasodilator Agents Aorta Thoracic Pharmacology Nitric Oxide Nitric oxide Glibenclamide chemistry.chemical_compound KATP Channels medicine.artery medicine Thoracic aorta Animals Rats Wistar Phenylephrine Antihypertensive Agents Stephania Aorta Tetraethylammonium Plant Extracts Vasodilation medicine.anatomical_structure Complementary and alternative medicine chemistry Hypertension Calcium Female Endothelium Vascular medicine.drug Phytotherapy |
Zdroj: | Journal of complementaryintegrative medicine. 12(1) |
ISSN: | 1553-3840 |
Popis: | Stephania abyssinica (DillonA. Rich) Walp (Menispermaceae) is a medicinal plant used in the west region of Cameroon to treat arterial hypertension. The present study evaluated the vasorelaxant effects of the aqueous (AESA) and methanol (MESA) extracts from the fresh leaves of S. abyssinica on aorta rings isolated from Wistar rats.Aorta rings with intact endothelium were contracted with KCl (60 mM) or phenylephrine (10-5 M) and exposed to cumulative concentrations of each extract, ranging from 10 to 1,000 µg/mL. The vasorelaxant effects of AESA were further evaluated in presence of Nω-nitro-L-arginine methyl ester (L-NAME, 10-4 M), tetraethylammonium (TEA, 5 µM), glibenclamide (5 µM), propranolol (5 µM), and the association glibenclamide-propranolol (AGP). In another set of experiments, the effect of AESA was evaluated on calcium-induced contraction in a hyperpotassic milieu.AESA and MESA exhibited a concentration-dependent vasorelaxation on KCl-contracted aortic rings with respective EC50 of 160.10 and 346.50 µg/mL. AESA similarly relaxed aortic rings contracted with phenylephrine (EC50, 176.80 µg/mL). The vasorelaxant activity of AESA was not significantly affected by L-NAME but was markedly reduced by TEA, glibenclamide, propranolol, and AGP. AESA strongly inhibited the Ca2+-induced contraction by 95%.These results support the use of S. abyssinica against arterial hypertension and suggest that the vasorelaxant effect of AESA is not mediated via the endothelium/nitric oxide pathway. AESA relaxant properties might be due to an inhibition of Ca2+ influx and/or the activation of ATP-sensitive K+ channels probably via the stimulation of β-adrenergic receptors. |
Databáze: | OpenAIRE |
Externí odkaz: |