Detection of SNPs in fish DNA: application of the fluorogenic ribonuclease protection (FRIP) assay for the authentication of food contents
| Autor: | Masahiro Goto, Momoko Kitaoka, Nobuko Okamura, Hirofumi Ichinose |
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| Rok vydání: | 2008 |
| Předmět: |
Fluorophore
Hot Temperature Food Handling Polymerase Chain Reaction Polymorphism Single Nucleotide law.invention chemistry.chemical_compound Ribonucleases law Food Labeling Fluorescence Resonance Energy Transfer Animals Nucleotide Ribonuclease Polymerase chain reaction chemistry.chemical_classification biology Tuna Fishes RNA General Chemistry DNA Anguilla Fluorescence Förster resonance energy transfer chemistry Biochemistry biology.protein General Agricultural and Biological Sciences |
| Zdroj: | Journal of agricultural and food chemistry. 56(15) |
| ISSN: | 1520-5118 |
| Popis: | The fluorogenic ribonuclease protection (FRIP) assay was used to detect single nucleotide polymorphisms (SNPs) in commercially produced fish products. By using fluorescence resonance energy transfer (FRET) between fluorophore and quencher labeled probes, the species-specific cleavage of sample RNA was detected by measuring the fluorescence intensity during the FRIP assay. We were able to discriminate raw and thermally processed eel and tuna species using the FRIP-based SNP detection method. Furthermore, the intensity of fluorescence was correlated with the mutant/wild-type ratio. These results suggest that the FRIP assay is a useful method for the in situ confirmation of labels of fishery foods during food production. |
| Databáze: | OpenAIRE |
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