Up-regulation of VLDL receptor expression and its signaling pathway induced by VLDL and beta-VLDL
| Autor: | Zong-Chen Feng, Yinghong Li, Yiqiang Zong, Yaozu Deng, He Li, Shen Qu, Yan Wang, Zhiguo Liu, Youmei Feng |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
MAPK/ERK pathway
Very low-density lipoprotein Cholesterol VLDL Biomedical Engineering VLDL receptor Very Low-Density Lipoprotein Receptor Biology Transfection digestive system Biochemistry Cell Line Biomaterials polycyclic compounds Genetics Transcriptional regulation Humans RNA Messenger Promoter Regions Genetic VLDLR Gene Protein kinase C Protein Kinase C Earth-Surface Processes Mitogen-Activated Protein Kinase 1 Macrophages nutritional and metabolic diseases Cell biology Up-Regulation Lipoproteins IDL Receptors LDL lipids (amino acids peptides and proteins) Signal transduction Signal Transduction |
| Zdroj: | Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 29(1) |
| ISSN: | 1672-0733 |
| Popis: | Very low density lipoprotein receptor (VLDLR) is thought to participate in the pathogenesis of atherosclerosis induced by VLDL and beta-VLDL. The present study was undertaken to elucidate the effects of VLDL and beta-VLDL on VLDLR expression and its signaling pathway. RAW264.7 cells were incubated with VLDL and beta-VLDL. The expression of VLDLR mRNA was detected by RT-PCR. The transcriptional activity of VLDLR gene was detected in recombinant plasmid pGL4.2VR-luciferase transfected RAW264.7. Western blot assay was used to detect the changes of phosphorylated ERK1/2 protein. Inhibitors or activators were used to observe the signal pathway involving VLDLR expression regulation. The results showed that VLDL and beta-VLDL stimulated ERK1/2 activity in a PKC-dependent manner. VLDL or beta-VLDL-induced VLDLR expression on macrophages was extremely abolished by inhibitors ERK1/2 or PKC. Our findings revealed that VLDL or beta-VLDL-induced VLDLR expression via PKC/ERK cascades and the effect was linked to the transcriptional activation of VLDLR gene promoter. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|