Bumble bee (Bombus impatiens) survival, pollen usage, and reproduction are not affected by oxalate oxidase at realistic concentrations in American chestnut (Castanea dentata) pollen
| Autor: | Dakota F Matthews, William A. Powell, Anastasia E Allwine, Scott H. McArt, Allison D. Oakes, Andrew E. Newhouse |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Pollination Oxalate oxidase media_common.quotation_subject Castanea dentata Flowers medicine.disease_cause 01 natural sciences Transgenic Bombus impatiens food Pollinator Chestnut blight Pollen Botany Genetics medicine Animals Biosafety media_common Risk assessment Original Paper biology GMO Reproduction fungi food and beverages Bees biology.organism_classification food.food 010602 entomology Animal Science and Zoology Oxidoreductases Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | Transgenic Research |
| ISSN: | 1573-9368 |
| Popis: | Transgenic American chestnut trees expressing a wheat gene for oxalate oxidase (OxO) can tolerate chestnut blight, but as with any new restoration material, they should be carefully evaluated before being released into the environment. Native pollinators such as bumble bees are of particular interest: Bombus impatiens use pollen for both a source of nutrition and a hive building material. Bees are regular visitors to American chestnut flowers and likely contribute to their pollination, so depending on transgene expression in chestnut pollen, they could be exposed to this novel source of OxO during potential restoration efforts. To evaluate the potential risk to bees from OxO exposure, queenless microcolonies of bumble bees were supplied with American chestnut pollen containing one of two concentrations of OxO, or a no-OxO control. Bees in microcolonies exposed to a conservatively estimated field-realistic concentration of OxO in pollen performed similarly to no-OxO controls; there were no significant differences in survival, bee size, pollen use, hive construction activity, or reproduction. A ten-fold increase in OxO concentration resulted in noticeable but non-significant decreases in some measures of pollen usage and reproduction compared to the no-OxO control. These effects are similar to what is often seen when naturally produced secondary metabolites are supplied to bees at unrealistically high concentrations. Along with the presence of OxO in many other environmental sources, these data collectively suggest that oxalate oxidase at field-realistic concentrations in American chestnut pollen is unlikely to present substantial risk to bumble bees. Supplementary Information The online version contains supplementary material available at 10.1007/s11248-021-00263-w. |
| Databáze: | OpenAIRE |
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