Turn On of a Ruthenium(II) Photocatalyst by DNA-Templated Ligation
| Autor: | Nicolas Winssinger, Marcello Anzola |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Peptide Nucleic Acids
Light Oligonucleotides chemistry.chemical_element 010402 general chemistry 01 natural sciences Catalysis Ruthenium chemistry.chemical_compound Bipyridine Coordination Complexes Humans Peptide nucleic acid Base Sequence 010405 organic chemistry Ligand Organic Chemistry Substrate (chemistry) General Chemistry DNA Combinatorial chemistry Fluorescence 0104 chemical sciences MicroRNAs chemistry Microscopy Fluorescence ddc:540 Photocatalysis MCF-7 Cells |
| Zdroj: | Chemistry-A European Journal, Vol. 25, No 1 (2019) pp. 334-342 |
| ISSN: | 1521-3765 0947-6539 |
| Popis: | Here, the synthesis of a RuII photocatalyst by light‐directed oligonucleotide‐templated ligation reaction is described. The photocatalyst was found to have tremendous potential for signal amplification with >15000 turnovers measured in 9 hours. A templated reaction was used to turn on the activity of this ruthenium(II) photocatalyst in response to a specific DNA sequence. The photocatalysis of the ruthenium(II) complex was harnessed to uncage a new precipitating dye that is highly fluorescent and photostable in the solid state. This reaction was used to discriminate between different DNA analytes based on localization of the precipitate as well as for in cellulo miRNA detection. Finally, a bipyridine ligand functionalized with two different peptide nucleic acid (PNA) sequences was shown to enable template‐mediated ligation (turn on of the ruthenium(II) photocatalysis) and recruitment of substrate for templated photocatalysis. |
| Databáze: | OpenAIRE |
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