Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-β1
| Autor: | Wei Wang, Xin Sun, Jianting Yao, Jiali Cheng, Jing Sun, Yuan-Qi Shi, Zengxiang Dong, Lu Wang, Zhen Tian, Yuanyuan Guo, Zhitao Li, Ye Tian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Physiology Cardiac fibrosis Intracellular Space Protoporphyrins Stimulation lcsh:Physiology Rats Sprague-Dawley 0302 clinical medicine Fibrosis TGF-β1 Ultrasonics lcsh:QD415-436 Phosphorylation SDT Cell Line Transformed Cell Death lcsh:QP1-981 Cell Cycle 030220 oncology & carcinogenesis Collagen medicine.medical_specialty Cell Survival Cardiac fibroblast Transforming Growth Factor beta1 lcsh:Biochemistry 03 medical and health sciences Internal medicine medicine Animals Smad3 Protein Protein kinase B Cell Proliferation Glycogen Synthase Kinase 3 beta business.industry Cell growth Myocardium AKT Sonodynamic therapy GSK3β Aminolevulinic Acid Fibroblasts medicine.disease 030104 developmental biology Endocrinology Animals Newborn Cell culture Cancer research business Proto-Oncogene Proteins c-akt Transforming growth factor |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 40, Iss 3-4, Pp 579-588 (2016) |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Background/Aims: Sonodynamic therapy (SDT) is a localized ultrasound-activated therapy for atherosclerosis when combined with a sonosensitizer, 5-aminolevulinic acid (ALA), but whether it can prevent cardiac fibrosis has not been studied. In the present study, we evaluated the effects SDT on fibrogenesis in rat cardiac fibroblasts. Methods: The primary cardiac fibroblasts were isolated from rats, and induced to fibrogenesis with TGF-β1. With this in vitro model, we tested the preventive effects of SDT on fibrogenesis and further the underlying mechanism. Results: TGF-β1 stimulation up-regulated α-SMA and COLI/III protein levels in cardiac fibroblasts, and enhanced the progression of cells from the G0/G1 phase to the S phase. SDT inhibited the TGF-β1 mediated cell proliferation and decreased the levels of α-SMA and COLI/III by activating AKT/GSK3β pathway and blocking TGF-β1/SMAD3 signaling. Conclusion: Our studies demonstrate an antifibrotic effect of SDT in rat cardiac fibroblasts, suggesting that SDT may intervene cardiac fibrogenesis by regulating myocardial fibrotic remodeling. |
| Databáze: | OpenAIRE |
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