Cedrol Enhances Extracellular Matrix Production in Dermal Fibroblasts in a MAPK-Dependent Manner
| Autor: | Young Ho Lee, Jeung Hoon Lee, Nam Ji Jeong, Mu Hyun Jin, Sun Gyoo Park, Minho Lee, Seok Seon Roh, Chang Deok Kim, Yul Lye Hwang |
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| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Annals of Dermatology |
| ISSN: | 2005-3894 1013-9087 |
| DOI: | 10.5021/ad.2012.24.1.16 |
| Popis: | Background: The extracellular matrix (ECM) produced by dermal fibroblasts supports skin structure, and degradation and/or reduced production of ECM are the main causes of wrinkle formation. Objective: The aim of this study was to identify the active ingredient that enhances ECM production in dermal fibroblasts. Methods: Polarity-based fractionation was used to isolate the active ingredient from natural extracts, and the effects of cedrol (isolated from Pterocarpus indicusirginia) on ECM production in cultured human dermal fibroblasts was investigated by reverse transcriptionpolymerase chain reaction, enzyme linked immunosorbent assay, and Western blot analysis. Results: Cedrol accelerated fibroblast growth in a dose-dependent manner and increased the production of type 1 collagen and elastin. Phosphorylation of p42/44 extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and Akt was markedly increased by cedrol, indicating that enhanced ECM production is linked to activation of intracellular signaling cascades. Conclusion: These results indicate that cedrol stimulates ECM production, with possible applications to the maintenance of skin texture. (Ann Dermatol 24(1) 16∼21, 2012) |
| Databáze: | OpenAIRE |
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