Acute effects of decreased glutamine supply on protein and amino acid metabolism in hepatic tissue: a study using isolated perfused rat liver
| Autor: | Ludek Sprongl, Milan Holecek, Jana Kadlcikova, Roman Safranek, Radana Rysava |
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| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Glutamine Protein metabolism Biology In Vitro Techniques O-Phthalaldehyde chemistry.chemical_compound Endocrinology Leucine Internal medicine medicine Animals Amino Acids Enzyme Inhibitors Rats Wistar Chromatography High Pressure Liquid chemistry.chemical_classification Catabolism Protein turnover Proteins Keto Acids Amino acid Rats Perfusion chemistry Biochemistry Liver Protein Biosynthesis Urea Oxidation-Reduction Algorithms Amino Acids Branched-Chain o-Phthalaldehyde Liver Circulation |
| Zdroj: | Metabolism: clinical and experimental. 52(8) |
| ISSN: | 0026-0495 |
| Popis: | Glutamine deficiency, a common finding in severe illness, has a negative influence on immune status, protein metabolism, and disease outcome. In several studies, a close relationship between glutamine, branched-chain amino acid (BCAA), and protein metabolism was demonstrated. The aim of the present study was to investigate the effect of glutamine deficiency on amino acid and protein metabolism in hepatic tissue using a model of isolated perfused rat liver (IPRL). Parameters of protein metabolism and amino acid metabolism were measured using both recirculation and single pass technique with L-[1-(14)C]leucine and [1-(14)C]ketoisocaproate (KIC) as a tracer. Glutamine concentration in perfusion solution was 0.5 mmol/L in control and 0 mmol/L in the glutamine-deficient group. The net release of glutamine (about 11 micromol/g/h) and higher net uptake of most of the amino acids was observed in the glutamine-deficient group. There was an insignificant effect of lack of glutamine on hepatic protein synthesis, proteolysis, and the release of urea. However, significantly lower release of proteins by the liver perfused with glutamine-deficient solution was observed. The lack of glutamine in perfusion solution caused a significant decrease in leucine oxidation (6.66 +/- 1.04 v 13.67 +/- 2.38, micromol/g dry liver/h, P |
| Databáze: | OpenAIRE |
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