Reduction in the level of intracellular myo-inositol in cultured soybean (Glycine max) cells inhibits cell division
| Autor: | David E. Hanke, M Biffen |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cell division
Chromatography Paper Cell growth Deoxyglucose Cell Biology Biology Biochemistry carbohydrates (lipids) Kinetics chemistry.chemical_compound chemistry Callus Glycine lipids (amino acids peptides and proteins) Inositol Soybeans Viability assay Molecular Biology Cell Division Cells Cultured Intracellular Research Article |
| Zdroj: | Biochemical Journal. 265:809-814 |
| ISSN: | 1470-8728 0264-6021 |
| Popis: | Although myo-inositol is included in media for the successful growth of plant tissues, the actual requirement of most tissues, including soybean (Glycine max) callus in suspension culture, for myo-inositol has not been demonstrated. We have made use of deoxyglucose to reduce intracellular levels of myo-inositol. Deoxyglucose is phosphorylated to deoxyglucose 6-phosphate, which inhibits L-myo-inositol 1-phosphate synthase, an important enzyme in the synthesis of myo-inositol. Addition of deoxyglucose to the medium resulted in a decrease in the intracellular level of myo-inositol that corresponded with a decrease in cell division. Cell viability was not affected. When myo-inositol was added to cells along with deoxyglucose, cell division was restored, as were intracellular levels of myo-inositol. Addition of myo-inositol had no affect on the uptake or metabolism of deoxyglucose. From these results we propose that myo-inositol has a role in maintaining cell division in soybean callus tissue in suspension culture. |
| Databáze: | OpenAIRE |
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