The parabrachial nucleus is a critical link in the transmission of short latency nociceptive information to midbrain dopaminergic neurons
| Autor: | Peter Redgrave, Eleanor J. Dommett, Véronique Coizet, Esther Marije Klop, Paul G. Overton |
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| Přispěvatelé: | Savasta, Marc, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Psychology [Sheffield], University of Sheffield [Sheffield], School of Life, Health and Chemical Sciences [Milton Keynes], Faculty of Science, Technology, Engineering and Mathematics [Milton Keynes], The Open University [Milton Keynes] (OU)-The Open University [Milton Keynes] (OU), Department of Neuroscience, University of Groningen [Groningen]-University Medical Centre - Anatomy Section, Wellcome Trust GR068021 Biotechnology and Biological Sciences Research Council BB/D019648/1 University of Sheffield Netherlands Organisation for Scientific Research |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
NHS normal horse serum Dopamine TX Triton X 100 PB phosphate buffer PPTg pedunculoponting tegmental nucleus MESH: Neurons Reticular formation 0302 clinical medicine Mesencephalon Pons RETICULAR-FORMATION rat MESH: Animals nociception Anesthetics Local MESH: GABA-A Receptor Antagonists ANOVA analysis of variance Neurons GLUTAMATERGIC NEURONS 0303 health sciences SUBSTANTIA-NIGRA MESH: Electrophysiology Chemistry Muscimol General Neuroscience Dopaminergic PHA-L Phaseolus vulgaris leukoagglutinin DA dopaminergic DORSAL-HORN NEURONS MESH: Electric Stimulation FOS-LIKE PROTEIN PBN parabrachial nucleus Ventral tegmental area Electrophysiology Substantia Nigra medicine.anatomical_structure extracellular recording LATERAL HABENULA Female [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] MESH: Pain SUPERIOR COLLICULUS Research Paper medicine.drug LAMINA-I NEURONS MESH: Rats Neuroscience(all) TH tyrosine hydroxylase MESH: Substantia Nigra Pain Substantia nigra MESH: Dopamine VTA ventral tegmental area MESH: Anesthetics Local DAB diaminobenzidine 03 medical and health sciences FLI fos-like immunoreactivity PBS phosphate buffered saline PSTH peri-stimulus time interval histogram medicine Animals GABA-A Receptor Antagonists [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] tract tracing 030304 developmental biology Parabrachial Nucleus Pars compacta SNPr substantia nigra pars reticulata NIGRA PARS COMPACTA Lidocaine MESH: Pons Pain Mechanisms and Sensory Neuroscience MESH: Mesencephalon BDA biotinylated dextran amine Electric Stimulation MESH: Male Rats VENTRAL TEGMENTAL AREA MESH: Lidocaine nervous system RMTg rostromedial tegmental nucleus SNPc substantia nigra pars compacta BSA bovine serum albumin SD standard deviation Neuroscience MESH: Female 030217 neurology & neurosurgery MESH: Muscimol |
| Zdroj: | Neuroscience Neuroscience, Elsevier-International Brain Research Organization, 2010, 168 (1), pp.263-72. ⟨10.1016/j.neuroscience.2010.03.049⟩ Coizet, V, Dommett, E J, Klop, E M, Redgrave, P & Overton, P G 2010, ' The parabrachial nucleus is a critical link in the transmission of short latency nociceptive information to midbrain dopaminergic neurons ', Neuroscience, vol. 168, no. 1, pp. 263-272 . https://doi.org/10.1016/j.neuroscience.2010.03.049 Neuroscience, 168(1), 263-272. PERGAMON-ELSEVIER SCIENCE LTD |
| ISSN: | 0306-4522 1873-7544 |
| DOI: | 10.1016/j.neuroscience.2010.03.049 |
| Popis: | Many dopaminergic neurons exhibit a short-latency response to noxious stimuli, the source of which is unknown. Here we report that the nociceptive-recipient parabrachial nucleus appears to be a critical link in the transmission of pain related information to dopaminergic neurons. Injections of retrograde tracer into the substantia nigra pars compacta of the rat labelled neurons in both the lateral and medial parts of the parabrachial nucleus, and intra-parabrachial injections of anterograde tracers revealed robust projections to the pars compacta and ventral tegmental area. Axonal boutons were seen in close association with tyrosine hydroxylase-positive (presumed dopaminergic) and negative elements in these regions. Simultaneous extracellular recordings were made from parabrachial and dopaminergic neurons in the anaesthetized rat, during the application of noxious footshock. Parabrachial neurons exhibited a short-latency, short duration excitation to footshock while dopaminergic neurons exhibited a short-latency inhibition. Response latencies of dopaminergic neurons were reliably longer than those of parabrachial neurons. Intra-parabrachial injections of the local anasethetic lidocaine or the GABA(A) receptor antagonist muscimol reduced tonic parabrachial activity and the amplitude (and in the case of lidocaine, duration) of the phasic response to footshock. Suppression of parabrachial activity with lidocaine reduced the baseline firing rate of dopaminergic neurons, while both lidocaine and muscimol reduced the amplitude of the phasic inhibitory response to footshock, in the case of lidocaine sometimes abolishing it altogether. Considered together, these results suggest that the parabrachial nucleus is an important source of short-latency nociceptive input to the dopaminergic neurons. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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