Recovery of protein from green leaves : Overview of crucial steps for utilisation
| Autor: | Govardus A.H. de Jong, Angélica Tamayo Tenorio, Jarno Gieteling, Remko M. Boom, Atze Jan van der Goot |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Dietary Fiber Ribulose-Bisphosphate Carboxylase Dry matter composition Leaf biorefinery Vegetable Proteins Fractionation Chemical Fractionation 01 natural sciences Plant Proteins Dietary Analytical Chemistry 0404 agricultural biotechnology 010608 biotechnology Pellet Protein purification Membrane proteins Centrifugation Protein extraction Solubility Food Process Engineering VLAG Chromatography biology Chemistry RuBisCO 04 agricultural and veterinary sciences General Medicine 040401 food science Plant Leaves Membrane protein biology.protein Beta vulgaris Food Science |
| Zdroj: | Food Chemistry 203 (2016) Food Chemistry, 203, 402-408 |
| ISSN: | 0308-8146 |
| Popis: | Plant leaves are a major potential source of novel food proteins. Till now, leaf protein extraction methods mainly focus on the extraction of soluble proteins, like rubisco protein, leaving more than half of all protein unextracted. Here, we report on the total protein extraction from sugar beet leaves (Beta vulgaris L.) by a traditional thermal extraction method consisting of mechanical pressing, heating to 50 °C and centrifugation. The resulting streams (i.e. supernatant, green-protein pellet and fibrous pulp) were characterised in terms of composition, physical structure and processing options. The protein distributed almost equally over the supernatant, pellet and pulp. This shows that thermal precipitation is an unselective process with respect to fractionation between soluble (rubisco) and insoluble (other) proteins. About 6% of the total protein could be extracted as pure rubisco (90% purity) from the supernatant. Surfactants commonly used for protein solubilisation could hardly re-dissolve the precipitated proteins in the pellet phase, which suggested that irreversible association was induced between the co-precipitated proteins and cell debris. Thus, the extraction of this protein will require prevention of their co-precipitation, and should take place in the original juice solution. |
| Databáze: | OpenAIRE |
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