A new xylanase from a Trichoderma harzianum strain
| Autor: | Adriane M. F. Milagres, M V de Sousa, C L de Medeiros, F Q de Paula Silveira, Edivaldo Ximenes Ferreira Filho, Carlos André Ornelas Ricart |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Trichoderma
biology Chemistry Size-exclusion chromatography Trichoderma harzianum Ultrafiltration Bioengineering Phenylalanine biology.organism_classification Applied Microbiology and Biotechnology Wood Glutamine Serine Molecular Weight Xylan Endo-1 3-beta-Xylosidase Xylosidases Kraft process Biochemistry Xylanase Chromatography Gel Electrophoresis Polyacrylamide Gel Food science Amino Acids Biotechnology Cysteine |
| Zdroj: | Journal of industrial microbiologybiotechnology. 23(1) |
| ISSN: | 1367-5435 |
| Popis: | A new xylanase (XYL2) was purified from solid-state cultures of Trichoderma harzianum strain C by ultrafiltration and gel filtration. SDS-PAGE of the xylanase showed an apparent homogeneity and molecular weight of 18 kDa. It had the highest activity at pH 5.0 and 45 degrees C and was stable at 50 degrees C and pH 5.0 up to 4 h xylanase. XYL2 had a low Km with insoluble oat spelt xylan as substrate. Compared to the amino acid composition of xylanases from Trichoderma spp, xylanase XYL2 presented a high content of glutamate/glutamine, phenylalanine and cysteine, and a low content of serine. Xylanase XYL2 improved the delignification and selectivity of unbleached hardwood kraft pulp. |
| Databáze: | OpenAIRE |
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