Antimycobacterial activity of selected medicinal plants traditionally used in Sudan to treat infectious diseases
| Autor: | Johanna Raffetseder, Henrik Andersson, Sadaf Kalsum, Nadir Abuzeid, Marie Larsson, Elsje Pienaar, Mikaela Glader, Waleed S. Koko, Haidar A. AlGadir, Richin John Koshy, Maria Lerm, M. Ahmed Mesaik, Muddathir S. Alhassan, Thomas Schön, Omer Mohamed Abdalla, Asaad Khalid, Daniel Eklund |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.drug_class
Antitubercular Agents Microbial Sensitivity Tests Antimycobacterial Rosmarinus Microbiology Mycobacterium tuberculosis Khaya Sudan Inhibitory Concentration 50 Drug Resistance Multiple Bacterial Drug Discovery medicine Humans Medicinal plants Medicine African Traditional Pharmacology Plants Medicinal biology Traditional medicine Dose-Response Relationship Drug Plant Extracts Macrophages biology.organism_classification visual_art Officinalis visual_art.visual_art_medium Bark Mycobacterium |
| Zdroj: | Journal of ethnopharmacology. 157 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance The emergence of multidrug-resistant strains of Mycobacterium tuberculosis underscores the need for continuous development of new and efficient methods to determine the susceptibility of isolates of Mycobacterium tuberculosis in the search for novel antimycobacterial agents. Natural products constitute an important source of new drugs, and design and implementation of antimycobacterial susceptibility testing methods are necessary to evaluate the different extracts and compounds. In this study we have explored the antimycobacterial properties of 50 ethanolic extracts from different parts of 46 selected medicinal plants traditionally used in Sudan to treat infectious diseases. Materials and methods Plants were harvested and ethanolic extracts were prepared. For selected extracts, fractionation with hydrophilic and hydrophobic solvents was undertaken. A luminometry-based assay was used for determination of mycobacterial growth in broth cultures and inside primary human macrophages in the presence or absence of plant extracts and fractions of extracts. Cytotoxicity was also assessed for active fractions of plant extracts. Results Of the tested extracts, three exhibited a significant inhibitory effect on an avirulent strain of Mycobacterium tubercluosis (H37Ra) at the initial screening doses (125 and 6.25 µg/ml). These were bark and leaf extracts of Khaya senegalensis and the leaf extract of Rosmarinus officinalis L. Further fractions of these plant extracts were prepared with n-hexane, chloroform, ethyl acetate, n-butanol, ethanol and water, and the activity of these extracts was retained in hydrophobic fractions. Cytotoxicity assays revealed that the chloroform fraction of Khaya senegalensis bark was non-toxic to human monocyte-derived macrophages and other cell types at the concentrations used and hence, further analysis, including assessment of IC50 and intracellular activity was done with this fraction. Conclusion These results encourage further investigations to identify the active compound(s) within the chloroform fraction of Khaya senegalensis bark. |
| Databáze: | OpenAIRE |
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