Glutamate and kainate receptors induced by rat brain messenger RNA in Xenopus oocytes
| Autor: | Ian Parker, Ricardo Miledi, C. B. Gundersen |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Drug Resistance
Xenopus Glutamic Acid Receptors Cell Surface Kainate receptor In Vitro Techniques Membrane Potentials Xenopus laevis Glutamates Receptors Kainic Acid Neurotransmitter receptor medicine Animals RNA Messenger Receptor Reversal potential General Environmental Science Messenger RNA Kainic Acid biology General Engineering Glutamate receptor Brain Rats Inbred Strains biology.organism_classification Molecular biology Rats Receptors Glutamate Protein Biosynthesis Oocytes Biophysics General Earth and Planetary Sciences Female Acetylcholine medicine.drug |
| Zdroj: | Proceedings of the Royal Society of London. Series B. Biological Sciences. 221:127-143 |
| ISSN: | 2053-9193 0080-4649 |
| DOI: | 10.1098/rspb.1984.0027 |
| Popis: | Xenopus laevis oocytes injected with poly(A) + mRNA extracted from rat brain became sensitive to serotonin, glutamate, kainate, acetylcholine and γ -aminobutyrate. Application of these substances to mRNA-injected oocytes elicited membrane currents. The glutamate- and acetylcholine-induced currents usually showed oscillations, while the kainate current was smooth. The current oscillations during glutamate application reversed direction at about the chloride equilibrium potential (— 24 mV), but the reversal potential for the kainate current was close to 0 mV. The current-voltage relation for the glutamate-induced current oscillations showed strong rectification at hyperpolarized potentials, while that for the kainate current was nearly linear. In some oocytes, glutamate elicited smooth membrane currents, with oscillations either absent, or appearing after a delay. The reversal potential of this component was close to 0 mV, and was clearly different from that of the oscillatory component. The appearance of glutamate and kainate sensitivity in the oocyte membrane is due to the translation of the foreign messenger RNA, and not to activation of the oocytes’ own genome, because oocytes still become sensitive when transcription is prevented by enucleation or by treatment with actinomycin D. It appears that mRNA from rat brain contains translationally active messengers which code for various neurotransmitter receptors. When this mRNA is injected into Xenopus oocytes, the messengers are translated and receptors are inserted into the oocyte membrane, where they form functionally active receptor-channel complexes. |
| Databáze: | OpenAIRE |
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