Performance of laterally elongated pillar array columns in capillary electrochromatography mode
| Autor: | Sertan Sukas, Heidi Ottevaere, Wim De Malsche, Pierre Philippe Gelin, Elahe Naghdi, Priya Laha, Agata Kryj, Herman Terryn, Martyna Baca |
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| Přispěvatelé: | Faculty of Engineering, Chemical Engineering and Industrial Chemistry, Materials and Chemistry, Electrochemical and Surface Engineering, Materials and Surface Science & Engineering, Applied Physics and Photonics, Brussels Photonics Team, Department of Bio-engineering Sciences, Centre for Molecular Separation Science & Technology, Chemical Engineering and Separation Science, Cell-Matrix Interact. Cardiov. Tissue Reg., Group Den Toonder, ICMS Affiliated |
| Rok vydání: | 2020 |
| Předmět: |
Central Zone
Materials science Clinical Biochemistry Pillar array columns 02 engineering and technology Kinetic energy 01 natural sciences Biochemistry Coumarin C440 Analytical Chemistry Capillary Electrochromatography Coumarins Lab-On-A-Chip Devices Dispersion (optics) Composite material CEC Capillary electrochromatography 010401 analytical chemistry Pillar Microfluidic chips Equipment Design 021001 nanoscience & nanotechnology Aspect ratio (image) language.human_language 0104 chemical sciences language Theoretical plate 0210 nano-technology |
| Zdroj: | ELECTROPHORESIS Electrophoresis, 41(15), 1287-1295. Wiley-VCH Verlag |
| ISSN: | 0173-0835 |
| DOI: | 10.1002/elps.202000001 |
| Popis: | In the present study, cylindrical and laterally elongated pillar array columns were investigated for use in capillary electrochromatography. Minimal theoretical plate heights of H = 1.90 and 1.46 μm (in absence of sidewall effect) were obtained for coumarin C440 under unretained conditions for cylindrical and rectangular (laterally elongated, aspect ratio 4) pillar array columns, respectively. By comparing dispersion at the entire channel width to that at the central zone only, it appears that sidewall related dispersion significantly contributes to overall dispersion. A 40% reduction of the plate height was observed by taking into account only the central channel zone. A kinetic plot analysis was performed to evaluate the potential of the studied geometries by considering a maximum operating voltage of 20 kV as limiting parameter. It was demonstrated that rectangular radially elongated pillars produce a higher efficiency than cylindrical pillars and other microfabricated column structures for microchip capillary electrochromatography previously studied. |
| Databáze: | OpenAIRE |
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