Engineering multiple species-like genetic incompatibilities in insects
| Autor: | Nathan Myslicki, Maciej Maselko, Michael B. O'Connor, Siba R. Das, Amanda N. Hayward, Michael J. Smanski, Aidan J. Peterson, Nathan Feltman, Ambuj Upadhyay |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
CRISPR-Cas9 genome editing
Male 0301 basic medicine Genotype Genetic Speciation Science Transgene 030231 tropical medicine General Physics and Astronomy Genes Insect Article General Biochemistry Genetics and Molecular Biology Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Genetic algorithm Melanogaster Animals Transgenes Allele lcsh:Science Drosophila Gene Synthetic biology Crosses Genetic Genetics Multidisciplinary Models Genetic biology fungi food and beverages General Chemistry Biocontainment biology.organism_classification Drosophila melanogaster 030104 developmental biology Hybridization Genetic lcsh:Q Female Genes Lethal Genetic Engineering |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-7 (2020) Nature Communications |
| DOI: | 10.1101/2020.04.03.024588 |
| Popis: | Speciation constrains the flow of genetic information between populations of sexually reproducing organisms. Gaining control over mechanisms of speciation would enable new strategies to manage wild populations of disease vectors, agricultural pests, and invasive species. Additionally, such control would provide safe biocontainment of transgenes and gene drives. Here, we demonstrate a general approach to create engineered genetic incompatibilities (EGIs) in the model insect Drosophila melanogaster. EGI couples a dominant lethal transgene with a recessive resistance allele. Strains homozygous for both elements are fertile and fecund when they mate with similarly engineered strains, but incompatible with wild-type strains that lack resistant alleles. EGI genotypes can also be tuned to cause hybrid lethality at different developmental life-stages. Further, we demonstrate that multiple orthogonal EGI strains of D. melanogaster can be engineered to be mutually incompatible with wild-type and with each other. EGI is a simple and robust approach in multiple sexually reproducing organisms. Natural speciation can be driven by pre-zygotic and post-zygotic barriers. Here the authors use a dominant lethal transgene coupled to a recessive resistance allele to engineer species-like barriers in Drosophila. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|