Cellular Distribution of a Colonic Adenoma-Associated Antigen as Defined by Monoclonal Antibody Adnab-9
| Autor: | S. Fugiel, M. S. Gesell, T. An, B. J. Maliakkal, Martin Tobi, V. Voruganti, James S. Hatfield, M. A. Alousi, R. H. Goo, Gordon D. Luk, M. Shafiuddin, S. Yang, V. Kaila |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Adenoma
Pathology medicine.medical_specialty Colon Colorectal cancer medicine.drug_class Blotting Western Enzyme-Linked Immunosorbent Assay Monoclonal antibody Antigen Antigens Neoplasm Biomarkers Tumor Tumor Cells Cultured Humans Medicine Intestinal Mucosa biology business.industry Gastroenterology Antibodies Monoclonal Cancer medicine.disease Colonic Neoplasms biology.protein Cancer research Immunohistochemistry Antibody Colorectal Neoplasms business Immunostaining |
| Zdroj: | Scandinavian Journal of Gastroenterology. 27:737-742 |
| ISSN: | 1502-7708 0036-5521 |
| DOI: | 10.3109/00365529209011175 |
| Popis: | Adenomatous colonic polyps constitute a precursor for colorectal cancer. Antibodies to these precancerous lesions might identify specific early tumor antigens. Adnab-9 is a murine monoclonal antibody raised against membranes of colonic adenomas. Adnab-9 binding in colonic washings (effluent) correlates with the presence of colorectal cancer. Immunohistochemical staining with Adnab-9 shows cytoplasmic reactivity in scattered cells in 4 of 31 adenomatous tissue sections, 0 of 14 sections of colorectal cancer cells, and 1 of 8 normal-appearing colonic mucosa specimens examined. Adnab-9 recognized a dominant M(r) 87,000 protein species in tissue extracts in the membrane-bound fraction of effluent by Western blotting. Adnab-9 binding by enzyme-linked immunosorbent assay in adenomatous extracts is higher than cancer or normal tissue, is membrane-bound, and is absent from established colorectal cancer cell lines. This distribution and nature of immunostaining suggest that Adnab-9 recognizes a determinant associated with the membrane component of a subpopulation of adenoma cells which may have a role in early colorectal neoplasia. |
| Databáze: | OpenAIRE |
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