Excitation–contraction coupling in resistance mesenteric arteries: Evidence for NKCC1-mediated pathway
| Autor: | Pavel Hamet, Johanne Tremblay, Svetlana V. Koltsova, Sergei N. Orlov, Sergei V. Kotelevtsev |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Vascular smooth muscle Sodium-Potassium-Chloride Symporters Biophysics Nitric Oxide Biochemistry Muscle Smooth Vascular Nitric oxide Mice chemistry.chemical_compound Sodium Potassium Chloride Symporter Inhibitors Internal medicine medicine Animals Solute Carrier Family 12 Member 2 Diuretics Molecular Biology Mesenteric arteries Phenylephrine Bumetanide biology Chemistry Depolarization Cell Biology Mesenteric Arteries Mice Inbred C57BL Vasodilation Nitric oxide synthase medicine.anatomical_structure Endocrinology Vasoconstriction Muscle Tonus biology.protein Vascular Resistance Cotransporter medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 379:1080-1083 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2009.01.018 |
| Popis: | Bumetanide and other high-ceiling diuretics (HCD) attenuate myogenic tone and contractions of vascular smooth muscle cells (VSMC) triggered by diverse stimuli. HCD outcome may be mediated by their interaction with NKCC1, the only isoform of Na(+), K(+), 2Cl(-) cotransporter expressed in VSMC as well as with targets distinct from this carrier. To examine these hypotheses, we compared the effect of bumetanide on contractions of mesenteric arteries from wild-type and NKCC1 knockout mice. In mesenteric arteries from wild-type controls, 100 microM bumetanide evoked a decrease of up to 4-fold in myogenic tone and contractions triggered by modest [K(+)](o)-induced depolarization, phenylephrine and UTP. These actions of bumetanide were preserved after inhibition of nitric oxide synthase with NG-nitro-l-arginine methyl ester, but were absent in mesenteric arteries from NKCC1(-/-) mice. The data show that bumetanide inhibits VSMC contractile responses via its interaction with NKCC1 and independently of nitric oxide production by endothelial cells. |
| Databáze: | OpenAIRE |
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