Neurotrophic effects of epidermal growth factor on cultured brain neurons are blocked by protein kinase inhibitors
| Autor: | Hiroshi Saito, Kazuho Abe, Megumi Takayanagi |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Cerebellum Cell Survival Carbazoles Hippocampus Biology Indole Alkaloids Alkaloids Epidermal growth factor Internal medicine medicine Staurosporine Animals Protein kinase A Cells Cultured Protein Kinase C Pharmacology Neurons Fetus Epidermal Growth Factor Brain Recombinant Proteins Cell biology Rats Endocrinology medicine.anatomical_structure Cell culture biology.protein hormones hormone substitutes and hormone antagonists medicine.drug Neurotrophin |
| Zdroj: | Japanese journal of pharmacology. 59(2) |
| ISSN: | 0021-5198 |
| Popis: | The influences of protein kinase inhibitors, K-252a and staurosporine, on the neuro-trophic effects of epidermal growth factor (EGF) were investigated in dissociated cell cultures of the hippocampus and cerebellum of fetal rats. Addition of 1 ng/ml EGF enhanced the survival of cultured neurons of both brain regions. Both K-252a (10-200 nM) and staurosporine (1-100 nM) blocked the survival-promoting effects of EGF in a concentration-dependent manner. These results suggest that activation of protein kinase(s) is involved in the neurotrophic effects of EGF. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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