Xanthene Dyes and Green LED for the Inactivation of Foodborne Pathogens in Planktonic and Biofilm States
| Autor: | Jane Martha Graton Mikcha, Andreia Farias Pereira Batista, Camila Fabiano de Freitas, Edinéia Bonin, Alex Fiori da Silva, Daliah Alves Coelho Trevisan, Adriele Rodrigues dos Santos, Noboru Hioka, Manuel Simões |
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| Přispěvatelé: | Faculdade de Engenharia |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Salmonella typhimurium
Salmonella Staphylococcus aureus 030303 biophysics medicine.disease_cause Biochemistry Microbiology 03 medical and health sciences chemistry.chemical_compound medicine Green led Rose bengal Physical and Theoretical Chemistry Coloring Agents Xanthene 0303 health sciences 030306 microbiology fungi Biofilm General Medicine Photosensitizing Agent Erythrosine chemistry Xanthenes Biofilms Food Microbiology Microscopy Electron Scanning |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | This study evaluated the rose bengal- and erythrosine-mediated photoinactivation against Salmonella Typhimurium and Staphylococcus aureus planktonic and sessile cells using green LED as a light source. The free-living or 2-day-old biofilm cells were treated with different concentrations of the photosensitizing agents and subjected to irradiation. Only 5 min photosensitization with rose bengal at 25 nmol L-1 and 75 mu mol L-1 completely eliminated S. aureus and S. Typhimurium planktonic cells, respectively. Erythrosine at 500 nmol L-1 and 5 min of light exposure also reduced S. aureus planktonic cells to undetectable levels. Eradication of S. aureus biofilms was achieved when 500 mu mol L-1 of erythrosine or 250 mu mol L-1 of rose bengal was combined with 30 min of irradiation. Scanning electron microscopy allowed the observation of morphological changes in planktonic cells and disruption of the biofilm architecture after photodynamic treatment. The overall data demonstrate that rose bengal and erythrosine activated by green LED may be a targeted strategy for controlling foodborne pathogens in both planktonic and sessile states. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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