Original Article. In vitro evaluation of potato genotypes for resistance against bacterial soft rot (Pectobacterium carotovorum) – a new tool for studying disease resistance
| Autor: | Cobra Moslemkhani, J. Mozafari, Nader Hasanzadeh, Sima Azadmanesh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine screening method Soil Science food and beverages Pectobacterium carotovorum in vitro Plant Science Biology Plant disease resistance lcsh:Plant culture biology.organism_classification 01 natural sciences Microbiology resistance 03 medical and health sciences Bacterial soft rot 030104 developmental biology Plant science Genotype lcsh:SB1-1110 potato soft rot Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Journal of Plant Protection Research, Vol 57, Iss 1, Pp 1-8 (2016) |
| Popis: | In vitro screening techniques were used to evaluate 46 genotypes of Iranian potato collection for resistance to bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc). One month old in vitro rooted potato plantlets were inoculated by two inoculation techniques under in vitro conditions: 1) sterile toothpicks dipped into bacterial suspension and pressed into the crown of plantlets and 2) the freshly cut crown of plantlets were dipped into bacterial suspension of 108 cfu ∙ ml-1 for 10 min. Typical soft rot disease symptoms, including the percentage of wilted leaves were recorded on inoculated plantlets 3, 6, 9, 12 and 15 days post-inoculation. The potato genotypes which were examined responded differently to Pcc and varying levels of resistance were observed. Potato genotype AG showed the highest level of resistance. Results obtained from in vitro screening were then verified by classical tuber slice assay. The verifications were conducted using five representative cultivars: Milva, Ramus, Picaso, Marfona and Agria which responded similarly to both in vitro and classical evaluation systems. Similar results obtained from these tests indicated that the in vitro screening technique developed in this study could provide a simple and rapid whole plant assay in selecting resistant potato genotypes against bacterial soft rot. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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