Optimized High-Performance Liquid Chromatographic Method for Determination of Lamotrigine in Serum With Concomitant Determination of Phenytoin, Carbamazepine, and Carbamazepine Epoxide
| Autor: | Gary L. Lensmeyer, Donald A. Wiebe, Barry E. Gidal |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pharmacology
Detection limit Chromatography Triazines Chemistry Elution medicine.medical_treatment Extraction (chemistry) Carbamazepine Lamotrigine High-performance liquid chromatography Anticonvulsant Phenytoin medicine Humans Anticonvulsants Pharmacology (medical) Solid phase extraction Quantitative analysis (chemistry) Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Therapeutic Drug Monitoring. 19:292-300 |
| ISSN: | 0163-4356 |
| DOI: | 10.1097/00007691-199706000-00009 |
| Popis: | Lamotrigine (LG), phenytoin (PY), carbamazepine (CM), and carbamazepine epoxide (CE) are measured with an optimized procedure that uses thin sorbent extraction disks and a highly selective, sterically protected bonded silica high-performance liquid chromatography (HPLC) column. Routinely, serum (200 microliters at pH 6.8 with cyheptamide as internal standard) is applied to an Empore octyl (C8) solid-phase extraction disk to isolate the drugs. a water wash removes interferences, and the retained drugs are eluted with a small volume of solvent. The eluate is directly injected onto a Zorbax Stable Bond cyanopropyl HPLC column with quantification at 214 nm. Evaporation-concentration steps are unnecessary. Overall, for all drugs, between-run precision coefficients of variation (n = 16 each) ranged from 2.1% to 4.9% at concentrations from 0.75 to 20.5 mg/l; extraction recoveries fell within a range of 96% to 110% at concentrations of 2, 10, and 30 mg/l tested for each drug; the lowest limit of detection was 0.15 to 0.35 mg/l. The analytical response was linear for each drug > 80 mg/l (LG) and > 50 mg/l (PY, CM, and CE). Optimization graphs are presented to illustrate the rationale for selection of test parameters for a robust method. In addition, a comparison study between two commercial laboratories demonstrates accuracy problems associated with LG testing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|