Platelet Adhesion to Multimeric and Dimeric von Willebrand Factor and to Collagen Type III Preincubated With von Willebrand Factor
| Autor: | Ya-Ping Wu, H. Lankhof, Robert I. Handin, Philip G. de Groot, Robert J. Wise, Jan J. Sixma, Hans H F I van Breugel |
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| Rok vydání: | 1996 |
| Předmět: |
Blood Platelets
congenital hereditary and neonatal diseases and abnormalities Time Factors Cations Divalent Prostaglandin Receptors Cell Surface CHO Cells Platelet Membrane Glycoproteins chemistry.chemical_compound Collagen Type III Platelet Adhesiveness Von Willebrand factor Cricetinae hemic and lymphatic diseases von Willebrand Factor medicine Animals Humans Platelet biology Heparin Chinese hamster ovary cell Osmolar Concentration Adhesion Epoprostenol Molecular biology chemistry Coagulation Biochemistry Microscopy Electron Scanning biology.protein Collagen Stress Mechanical Cardiology and Cardiovascular Medicine circulatory and respiratory physiology medicine.drug |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 16:611-620 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.16.5.611 |
| Popis: | Abstract As part of a systematic study of platelet interaction with adhesive proteins under flow conditions, we studied platelet adhesion to multimeric and dimeric von Willebrand factor (vWF) coated to glass. vWF-dependent adhesion to collagen type III was studied for comparison. Adhesion to glass-coated vWF and vWF-mediated adhesion to collagen type III were in many respects similar. Both showed no decrease at increasing shear rates and a decline to 50% of maximum with a low-molecular-weight multimeric fraction. Adhesion to glass-coated vWF was partially inhibited by heparin and completely inhibited by prostaglandin I 2 and anti–glycoprotein (GP) Ib and anti–GPIIb-IIIa antibodies. vWF-dependent adhesion to collagen was not inhibited by heparin, was partially inhibited by anti–GPIIb-IIIa, and was completely inhibited by prostaglandin I 2 and anti-GPIb. Recombinant dimeric vWF was made by deletion of the propeptide and expression in Chinese hamster ovary cells. Adhesion was 50% of that with plasma vWF, and larger concentrations of dimeric vWF were required. Adhesion to dimeric vWF was optimal at 1500 s −1 , with a gradual decrease at higher shear rates. We conclude that adhesion to collagen type III is strongly but not completely determined by the adhesive properties of vWF. |
| Databáze: | OpenAIRE |
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