Cytoplasmic pH in the action of insulin-like growth factor-I in cultured porcine thyroid cells
| Autor: | Y. Nagasawa, Nobuyuki Takasu, Yoshifusa Shimizu, Takayuki Asawa, I. Komiya, Takashi Yamada |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cytoplasm
medicine.medical_specialty Swine Endocrinology Diabetes and Metabolism medicine.medical_treatment Thyroid Gland Biology Amiloride Insulin-like growth factor chemistry.chemical_compound Endocrinology Epidermal growth factor Internal medicine medicine Animals Insulin-Like Growth Factor I Cells Cultured Growth factor Sodium Thyroid Hydrogen-Ion Concentration Nerve growth factor medicine.anatomical_structure chemistry Cell culture Thymidine Cell Division Endocrine gland |
| Zdroj: | Journal of Endocrinology. 127:305-309 |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1677/joe.0.1270305 |
| Popis: | The effects of insulin-like growth factor-I (IGF-I) on cytoplasmic pH (pHi) and [3H]thymidine incorporation were studied in primary cultures of porcine thyroid cells. IGF-I alkalinized thyroid cells and stimulated thymidine incorporation in a dose-dependent manner; the effects of IGF-I on alkalinization (the maximal rates of change of cytoplasmic pHi/min ((dpHi/dt)max)) and thymidine incorporation were observed at 2 ng/ml and were maximal at 100 ng/ml, with half-maximal stimulation at approximately 10 ng/ml. The results indicate that Na+/H+ exchange or cell alkalinization may function as a transmembrane signal transducer in the action of IGF-I on thyroid cell proliferation. Several mitogens and comitogens which activate sodium hydrogen exchange, including epidermal growth factor, platelet-derived growth factor and nerve growth factor, have been listed. Activation with IGF-I has not, however, been presented before. Thus the present study constitutes the first demonstration of IGF-I-stimulated activation of Na+/H+ exchange or cell alkalinization. Journal of Endocrinology (1990) 127, 305–309 |
| Databáze: | OpenAIRE |
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