The effects of age and various fat/carbohydrate caloric ratios on nitrogen retention and wound healing in rats
| Autor: | Jason G. Nirgiotis, Richard J. Andrassy, Patrick J. Hennessey |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Nitrogen balance Aging Nitrogen Weanling chemistry.chemical_element Enteral administration Animal science medicine Dietary Carbohydrates Animals Serum Albumin Analysis of Variance Wound Healing business.industry Rats Inbred Strains General Medicine Metabolism Blood Proteins Carbohydrate Dietary Fats Surgery Rats chemistry Pediatrics Perinatology and Child Health Collagen Energy source business Wound healing |
| Zdroj: | Journal of pediatric surgery. 26(4) |
| ISSN: | 0022-3468 |
| Popis: | Recent reports have indicated a possible age-related component to the ability of various species to utilize either fat or carbohydrate as their preferential energy source. These studies have demonstrated that infant rats given a high-fat diet (50% fat, 35% carbohydrate, 15% protein) retain a significantly higher amount of nitrogen than do infant rats on a high-carbohydrate diet (82% carbohydrate, 3% fat, 15% protein). Conversely, adult rats maintain a higher positive nitrogen balance when given the high-carbohydrate diet. In light of these studies, and our interest in factors that contribute to wound healing in the surgical patient, we investigated the effects of low-, medium-, and high-fat enteral diets on wound healing and nitrogen balance in surgically stressed rats of various ages. Weanling (45 g) and young adult (175 g) male Sprague-Dawley rats (n = 16 per age group) were housed individually in metabolic cages. All animals had Impravascular-graft wound cylinders placed subcutaneously in the dorsal midline and were placed in four dietary groups: high fat (50% kcal), medium fat (30% kcal), low fat (2.5% kcal), and chow (Purina Standard Laboratory Rodent Chow, 17% kcal as fat). All test diets were isonitrogenous (3.1 g nitrogen/1,000 kcal) and isocaloric for each age group. Animals in each age group were pair-fed (35 kcal/d, weanlings; 60 kcal/d, young adults) for 10 days and then euthanized. Wound cylinders were removed and analyzed for collagen content. Weanling animals fed the high-fat diet retained significantly more daily nitrogen (89% ± 5% v 18% ± 8% of daily enteral nitrogen, P P P |
| Databáze: | OpenAIRE |
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